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Gibco™ DMEM, high glucose, pyruvate DFS Item

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, Pyruvate is used in cell culture processing applications.

Manufacturer:  Gibco™ 11995065

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Catalog No. 11995065



Requires : Supplementation

Description

Description

  • Contains four times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12
Specifications

Specifications

1X
Sterile-filtered
Room Temperature
Gibco™
L-Glutamine
12 Months From Date of Manufacture
500mL
High Glucose, L-Glutamine, Phenol Red, Sodium Pyruvate
High Glucose
Mammalian Cell Culture
Animal Origin-Free
No HEPES
DMEM (Dulbecco's Modified Eagle Medium)
Standard Serum Supplementation
500 ml
Liquid
Phenol Red
Sustainable packaging
primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells
HEPES
Sodium Pyruvate
HeLa, 293, Cos-7, and PC-12
DMEM (Dulbecco's Modified Eagle Medium)
SDS
Documents
Product Certifications
Promotions

Promotions

When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.