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Gibco™ DMEM, high glucose, no glutamine DFS Item

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, No Glutamine is used in cell culture processing applications.

Manufacturer:  Gibco™ 11960069

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Catalog No. 11960069

Requires : Supplementation



  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12


Room Temperature
No Glutamine
12 Months From Date of Manufacture
10 x 500mL
High Glucose, Phenol Red
High Glucose
HeLa, 293, Cos-7, and PC-12
DMEM (Dulbecco's Modified Eagle Medium)
DMEM (Dulbecco's Modified Eagle Medium)
Standard Serum Supplementation
500 ml
Phenol Red
Sustainable packaging
primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells
L-Glutamine, Sodium Pyruvate, HEPES
No Sodium Pyruvate
Animal Origin-Free
Product Certifications


When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.