Thermo Scientific™ CaptureSelect™ LambdaXP Affinity Matrix

Features of this affinity matrix include:

• Efficient elution of Fab fragments and immunoglobulins containing a lambda light chain, no cross binding to the Fc-region or kappa light chains
• One-step purification from crude material with high purity and yield
• Excellent scalability
• Non-animal-derived

CaptureSelect LambdaXP Affinity Matrix can be utilized for the purification of human Ig's and Fab/Fab2 fragments directly from complex source materials and enables a robust and efficient purification process with excellent purity obtained in a single step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: agarose-based, epoxide-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect LambdaXP
• Ligand coupling method: epoxide coupling
• Binding capacity: >30 g IgG/L resin depending on flow rate, column height, and contact time
• Elution conditions: 25 mM sodium acetate, pH 3.5
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol