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Protein Purification Reagents and Kits

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1

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Product Line HisPur
2

Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 3 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

3

Thermo Scientific™ Pierce™ High-Capacity EDTA-Compatible Ni-IMAC Resin

Pierce High-Capacity EDTA-Compatible Ni-IMAC Resin is designed for the purification of over-expressed His-tagged proteins from cell culture media.

Binding Property Chelator Stable
4

Thermo Scientific™ HisPur™ Cobalt Spin Columns, 3 mL

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

5

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Pierce Anti-DYKDDDDK Spin Columns provide a fast, convenient method for purification of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells.

Shipping Condition Wet Ice
Stationary Phase Anti-DYKDDDDK monoclonal antibodies
Product Type Affinity Resin
6

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

7

Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide

Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports

Shipping Condition Wet Ice
Matrix Affinity Resin
Format Lyophilized Powder
Form Lyophilized Powder
Protein Form Recombinant
Product Type Peptides
For Use With (Application) Protein Purification and Isolation
Product Line Pierce
8
Promotions Available

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (Multi-species) Conjugate
GREENER_CHOICE

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (ms) Conjugate consists of a 13 kDa recombinant single domain antibody fragment that specifically binds to the Fc part of IgG from multiple species.

Ligand Type Antibody
Type Conjugate
Target IgG (Fc region)
Content And Storage Store at -5°C to -30°C.
Form Liquid
Label or Dye Biotin & Other Haptens
Product Line CaptureSelect
9

Cytiva HiScreen™ Capto™ S, Capto™ Q and Capto™ DEAE Prepacked IEX Columns

Designed for method optimization and parameter screening

Buffer 1M Acetic Acid, 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Column Type Ion Exchange Column
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Modern large-scale manufacturers for fast, efficient and cost-effective capture and intermediate Protein purification, optimization and parameter screening
Max. Pressure 3 bar
Particle Size 45 to 165μm
Flow Rate <700 cm/hr.
Support Type Highly cross-linked Agarose with Dextran surface extender
Height (English) 3.93 in.
10

Invitrogen™ eBioscience™ Protein Transport Inhibitor Cocktail (500X)

Suitable for use in assays in which prevention of protein transport to the extracellular space is desired.

Type Protein Transport Inhibitor Cocktail
Product Line eBioscience
11

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
12

Cytiva HiTrap Capto MMC Columns

HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

Particle Size 75μm
Buffer Aqueous Buffer
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 2 to 12
Product Type HiTrap Capto MMC column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Bind Proteins at the conductivity of the feed material and to solve specific purification challenges
Support Type Highly Cross-linked Agarose
Height (English) 0.98 in.
13

Cytiva LabMate™ PD-10 Buffer Reservoirs

Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. Cytiva Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.

14

Thermo Scientific™ AminoLink™ Immobilization Kit

Covalently immobilize proteins and antibodies by lysine residues or other primary amines (-NH2) via reductive amination with these aldehyde-activated agarose beads.

15

Thermo Scientific™ CaptureSelect™ CH1-XL Pre-packed Column

Thermo Scientific™ CaptureSelect™ CH1-XL Affinity Matrix is an improved version of CaptureSelect IgG-CH1 Affinity Matrix, featuring a wider elution range (pH 3 to 4.5), increased binding capacity, and improved stability at higher pH, offered here in pre-packed format.

Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Ligand Type CH1-XL ligand
Stationary Phase CH1-XL
Type Pre-packed Column
Content And Storage Store in refrigerator (2°C to 8°C).
Product Line CaptureSelect