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Applied Biosystems™ Fast SYBR™ Green Master Mix
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
Supplier: Applied Biosystems™ 4385614
Includes: SYBR Green I Dye, AmpliTaq Gold Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference I and optimized buffer components
Requires : Template and primers
Description
- A fast, reliable, and cost-effective solution for real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency
- Real-time PCR results in as fast as 35 minutes
- Capable of detecting very low copies of target
- Minimizes primer-dimer and non-specific amplification
- Consistent amplification across a wide dynamic range
- Contains all of the components, excluding the template and primers, in a convenient 2X master mix
- Store at -5°C to -30°C
Includes the following components in an optimized buffer:
- AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start
- Minimizes non-specific product formation and enables reactions to be set up at room temperature
- SYBR™ Green I dye to enable detection of double-stranded DNA
- Deoxynucleotides (dNTPs) to help maintain optimal PCR results
- Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
- Passive internal reference based on proprietary ROX™ dye to enable increased precision
Order Info
Shipping Condition: Dry Ice
Chemical Identifiers
| 2X | |
| High | |
| AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP | |
| 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode | |
| ROX (Pre-mixed) | |
| Real Time PCR SYBR Master Mix | |
| Gene Expression |
| SYBR | |
| qPCR | |
| Fast | |
| SYBR Green | |
| SYBR | |
| UP (Ultra Pure) | |
| DNA (Genomic), cDNA |
Specifications
| 2X | |
| SYBR | |
| High | |
| AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP | |
| 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode | |
| ROX (Pre-mixed) | |
| Real Time PCR SYBR Master Mix | |
| 1 x 50 mL | |
| 5000 Reactions of 20 μL Each | |
| 5000 Reactions | |
| 50 mL |
| Contains 1 × 50 mL vial. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Sufficient for 5000 reactions of 20 μL each. Store at -5 to -30°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
|
| Vial | |
| qPCR | |
| Fast | |
| SYBR Green | |
| SYBR | |
| UP (Ultra Pure) | |
| Dry Ice | |
| Gene Expression | |
| DNA (Genomic), cDNA |
For Research Use Only. Not for use in diagnostic procedures.