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Applied Biosystems™ Fast SYBR™ Green Master Mix DFS Item

Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

Supplier:  Applied Biosystems™ 4385618

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Catalog No. 4385618



  • A fast, reliable, and cost-effective solution for real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency
  • Real-time PCR results in as fast as 35 minutes
  • Capable of detecting very low copies of target
  • Minimizes primer-dimer and non-specific amplification
  • Consistent amplification across a wide dynamic range
  • Contains all of the components, excluding the template and primers, in a convenient 2X master mix
  • Store at -5°C to -30°C

Includes the following components in an optimized buffer:

  • AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start
    • Minimizes non-specific product formation and enables reactions to be set up at room temperature
  • SYBR™ Green I dye to enable detection of double-stranded DNA
  • Deoxynucleotides (dNTPs) to help maintain optimal PCR results
  • Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
  • Passive internal reference based on proprietary ROX™ dye to enable increased precision


10 × 5 mL vials. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1, and optimized buffer components. Sufficient for 5000 reactions of 20 μL each.

Store at -5 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

SYBR Green
UP (Ultra Pure)
5000 Reactions of 20 μL Each
DNA (Genomic), cDNA
AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
ROX (Pre-mixed)
Real Time PCR SYBR Master Mix
10 x 5 mL
Gene Expression
5000 Reactions
10 x 5 mL




For Research Use Only. Not for use in diagnostic procedures.