missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Real Time PCR Products
  6. Real Time PCR Master Mixes
  7. Applied Biosystems™ Fast SYBR™ Green Master Mix

Applied Biosystems™ Fast SYBR™ Green Master Mix

Catalog No. 4385610
Encompass
Change view
Click to view available options
Quantity:
1 x 1 mL
1 x 5 mL
2 x 5 mL
5 x 5 mL
10 x 5 mL
1 x 50 mL
6 product options available for selection
Product selection table with 6 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
4385610 1 x 1 mL
4385612 1 x 5 mL
4385616 2 x 5 mL
4385617 5 x 5 mL
4385618 10 x 5 mL
4385614 1 x 50 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 6 options available.
6 options
Catalog No. 4385610 Supplier Applied Biosystems™ Supplier No. 4385610
Only null left

Description

Includes

SYBR Green I Dye, AmpliTaq Gold Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference I and optimized buffer components

Requires

Template and primers

Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

  • A fast, reliable, and cost-effective solution for real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency
  • Real-time PCR results in as fast as 35 minutes
  • Capable of detecting very low copies of target
  • Minimizes primer-dimer and non-specific amplification
  • Consistent amplification across a wide dynamic range
  • Contains all of the components, excluding the template and primers, in a convenient 2X master mix
  • Store at -5°C to -30°C

Includes the following components in an optimized buffer:

  • AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start
    • Minimizes non-specific product formation and enables reactions to be set up at room temperature
  • SYBR™ Green I dye to enable detection of double-stranded DNA
  • Deoxynucleotides (dNTPs) to help maintain optimal PCR results
  • Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
  • Passive internal reference based on proprietary ROX™ dye to enable increased precision

Order Info

Shipping Condition: Dry Ice

Specifications

Concentration 2X
Content And Storage 1 x 1 mL vial. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1 and optimized buffer components. 100 reactions in a 20 μL total volume. Store at -5 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method SYBR
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Reaction Speed Fast
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Label or Dye SYBR Green
Passive Reference Dye ROX (Pre-mixed)
Product Line SYBR
Product Type Real Time PCR SYBR Master Mix
Purity or Quality Grade UP (Ultra Pure)
Quantity 1 x 1 mL
Shipping Condition Dry Ice
Sufficient For 100 Reactions of 20 μL
For Use With (Application) Gene Expression
No. of Reactions 100 Reactions
Sample Type DNA (Genomic), cDNA
Volume 1 mL
Show More Show Less

Frequently Asked Questions (FAQs)

Is there a SYBR Green Fast Master Mix currently available?

Yes. The following reagents can be used for fast cycling: Fast SYBR Green Master Mix, EXPRESS SYBR GreenER qPCR Supermix Universal, and SYBR GreenER qPCR SuperMix for ABI PRISM instruments.

For Research Use Only. Not for use in diagnostic procedures.