HPLC Columns
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MilliporeSigma™ Supelco™ Discovery™ C18 HPLC Column, 5 μm Particle Size
Use Discoveryâ„¢ C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.
| Pore Size | 180 Ã… |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | C18 (Octadecyl) |
| Phase | Reversed Phase |
| Matrix | C18 (Octadecyl) Phase/Silica Gel, High Purity, Spherical Particle Platform |
| Endcapped | Yes |
| pH | 2 to 8 |
| Material | Stainless Steel |
| Surface Area | 200 m2/g |
| Product Line | Discovery |
MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C5 HPLC Column, 10 μm Particle Size
Discoveryâ„¢ BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC.
| Pore Size | 300 Ã… |
|---|---|
| Particle Size | 10 μm |
| Stationary Phase | C5 (pentyl) |
| Phase | Reversed Phase |
| Matrix | C5 (pentyl) Phase/Silica Particle Platform |
| Endcapped | Yes |
| Technique | Wide Pore HPLC |
| Surface Area | 100 m2/g |
| Product Line | Discovery |
Thermo Scientificâ„¢ Acclaimâ„¢ C30 HPLC Columns
Use for superior separation of hydrophobic structural isomers.
| Pore Size | 200 Ã… |
|---|---|
| Carbon Load | 13% |
| Stationary Phase | C30 |
| Temperature | 60°C |
| Phase | Reversed Phase |
| Column Type | Reversed Phase |
| pH | 2 to 8 |
| Surface Area | 200 m²/g |
| Product Line | Acclaim |
Thermo Scientificâ„¢ Syncronisâ„¢ C18 Columns
Use for hydrophobic interaction reversed-phase separations.
| Pore Size | 100 Ã… |
|---|---|
| Carbon Load | 16% |
| Stationary Phase | C18 |
| Temperature | 60°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| pH | 2 to 9 |
| Surface Area | 320 m²/g |
| USP Type | L1 |
TSKgel™ Butyl-NPR HPLC Column, 2.5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgelâ„¢ Butyl-NPR columns are not as hydrophobic, i.e., less retentive, than Phenyl-5PW and Ether-5PW HIC columns. They are the best choice for high speed separations and provide excellent recovery even for more hydrophobic proteins.
MilliporeSigma Ascentisâ„¢ Express PCS-C18 HPLC Column
Ascentisâ„¢ Express PCS-C18 columns are specifically engineered to provide efficient separation of basic, acidic, or neutral compounds under low ionic strength (formic acid) mobile phase conditions.
| Pore Size | 90 Ã… |
|---|---|
| Particle Size | 2.7 μm |
| Type | HPLC Column |
| Phase | Positively Charged Surface (PCS) |
| Material | Stainless Steel |
| Technique | HPLC |
MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 10 μm Particle Size
The Discoveryâ„¢ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
| Pore Size | 300 Ã… |
|---|---|
| Particle Size | 10 μm |
| Stationary Phase | C18 (octadecyl) |
| Phase | Reversed Phase |
| Matrix | C18 (octadecyl) Phase/silica particle platform |
| Endcapped | Yes |
| Technique | Wide Pore HPLC |
| Surface Area | 100 m2/g |
| Product Line | Discovery |
Thermo Scientificâ„¢ Accucoreâ„¢ 150-C4 HPLC Columns
Use this specially designed solid core column with a C4 functionality for biomolecules that bind too tightly to C8 or C18 columns.
| Pore Size | 150 Ã… |
|---|---|
| Particle Size | 2.6 μm |
| Carbon Load | 2% |
| Stationary Phase | C4 |
| Temperature | 60°C |
| Phase | Reversed Phase |
| For Use With | HPLC |
| Endcapped | Yes |
| Packing Material | Spherical, Solid Core, Ultrapure Silica |
| Column Type | Reversed Phase |
| pH | 2 to 9 |
| Surface Area | 80 m²/g |
| USP Type | L26 |
Thermo Scientificâ„¢ Accucoreâ„¢ Phenyl-Hexyl HPLC Columns
Use this solid core column for fast, high-resolution separations of aromatic and moderately polar compounds.
| Pore Size | 80 Ã… |
|---|---|
| Temperature | 70°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| Surface Area | 130 m²/g |
| Max. Pressure | 14500 psi (1000 bar) |
| Particle Size | 2.6 μm |
| Carbon Load | 5% |
| Packing Material | Spherical, Solid Core, Ultrapure Silica |
| pH | 2 to 8 |
| Particle Shape | Spherical Solid Core |
| Product Line | Accucore |
| USP Type | L11 |
Thermo Scientific™ Aquasil™ 5μm C18 HPLC Columns, 5?m Particle Size
Thermo ScientificTM AquasilTM 5μm C18 HPLC Columns are available in limited dimensions. Please contact Customer Service for more details.
| Pore Size | 100 Ã… |
|---|---|
| Particle Size | 5 μm |
| Carbon Load | 12% |
| Stationary Phase | C18 |
| Phase | Reversed Phase |
| For Use With | LC/MS |
| Endcapped | Yes |
| Packing Material | Silica |
| Column Type | Reversed Phase |
| pH | 2 to 8 |
| Surface Area | 310 m²/g |
| USP Type | L1 |
| Column Format | Analytical Column |
Thermo Scientificâ„¢ Acclaimâ„¢ Polar Advantage II HPLC Columns
Resolve polar and nonpolar compounds in a single run. These columns have high pH stability (pH 1.5–10) in the separation of basic, neutral, and acidic species.
| Pore Size | 120 Ã… |
|---|---|
| Stationary Phase | Polar Embedded |
| Temperature | 60°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Packing Material | Spherical, Fully Porous, Ultrapure Silica |
| Column Type | Reversed Phase |
| Surface Area | 300 m²/g |
| USP Type | L60 |
MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 2.7 μm Particle Size
Ascentisâ„¢ Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Restek™ Pinnacle™ DB C8 Specialty/Application Specific HPLC Column, 5 μm
Base-deactivated spherical silica optimized for UHPLC stability.
| Pore Size | 140 Ã… |
|---|---|
| Stationary Phase | C8 |
| Temperature | 80°C |
| Phase | Reversed Phase |
| For Use With | Analysis of Basic Compounds or Bases Mixed with Acids/Neutrals |
| Endcapped | Yes |
| Column Type | Specialty/Application Specific |
| Quantity | 5 g |
| Material | Stainless Steel |
| Max. Pressure | 4000 psi (275 bar) |
| Particle Size | 5 μm |
| Ligand Type | Monomeric C8 |
| Carbon Load | 6% |
| Packing Material | Silica |
| pH | 2.5 to 10 |
| Technique | UHPLC |
| Particle Shape | Spherical |
| Film Thickness | 5 μm |
| USP Type | L7 |
Restek™ Viva™ HPLC Columns with C18 Column Packing, 5μm Particle Size
Wide bore silica columns excellent for general-purpose applications and separating peptides or proteins
| Pore Size | 300 Ã… |
|---|---|
| Stationary Phase | C18 |
| Temperature | 80°C |
| Phase | Reversed Phase |
| For Use With | Analyzing Large Molecules and Biomolecules |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| Particle Size | 5 μm |
| Type | Column |
| Carbon Load | 9% |
| Packing Material | Silica |
| pH | 2.5 to 10 |
| Particle Shape | Spherical |
| Film Thickness | 5 μm |
| USP Type | L1 |
| Pore Size | 100 Ã… |
|---|---|
| Type | Cartridge |
| Stationary Phase | C18 |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Packing Material | Silica |
| Column Type | Reversed Phase |
| Material | Stainless Steel |
| USP Type | L1 |