HPLC Columns
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MilliporeSigma™ Supelco™ Discovery™ C18 HPLC Column, 5 μm Particle Size
Use Discovery™ C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.
| Phase | Reversed Phase |
|---|---|
| Matrice | C18 (Octadecyl) Phase/Silica Gel, High Purity, Spherical Particle Platform |
| Matériau | Stainless Steel |
| “End-capped” | Yes |
| Gamme de produits | Discovery |
| pH | 2 to 8 |
| Surface spécifique | 200 m2/g |
| Granulométrie | 5 μm |
| Porosité | 180 Å |
| Phase stationnaire | C18 (Octadecyl) |
MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C5 HPLC Column, 10 μm Particle Size
Discovery™ BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC.
| Phase | Reversed Phase |
|---|---|
| Matrice | C5 (pentyl) Phase/Silica Particle Platform |
| “End-capped” | Yes |
| Gamme de produits | Discovery |
| Surface spécifique | 100 m2/g |
| Granulométrie | 10 μm |
| Technique | Wide Pore HPLC |
| Porosité | 300 Å |
| Phase stationnaire | C5 (pentyl) |
TSKgel™ Butyl-NPR HPLC Column, 2.5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ Butyl-NPR columns are not as hydrophobic, i.e., less retentive, than Phenyl-5PW and Ether-5PW HIC columns. They are the best choice for high speed separations and provide excellent recovery even for more hydrophobic proteins.
Thermo Scientific™ Syncronis™ C18 Columns
Use for hydrophobic interaction reversed-phase separations.
| Phase | Reversed Phase |
|---|---|
| Température | 60°C |
| Taux de carbone | 16% |
| “End-capped” | Yes |
| Type d’USP | L1 |
| pH | 2 to 9 |
| Surface spécifique | 320 m²/g |
| Porosité | 100 Å |
| Type de colonne | Reversed Phase |
| Phase stationnaire | C18 |
MilliporeSigma Ascentis™ Express PCS-C18 HPLC Column
Ascentis™ Express PCS-C18 columns are specifically engineered to provide efficient separation of basic, acidic, or neutral compounds under low ionic strength (formic acid) mobile phase conditions.
| Type | HPLC Column |
|---|---|
| Phase | Positively Charged Surface (PCS) |
| Matériau | Stainless Steel |
| Granulométrie | 2.7 μm |
| Technique | HPLC |
| Porosité | 90 Å |
Thermo Scientific™ Acclaim™ C30 HPLC Columns
Use for superior separation of hydrophobic structural isomers.
| Phase | Reversed Phase |
|---|---|
| Température | 60°C |
| Taux de carbone | 13% |
| Gamme de produits | Acclaim |
| pH | 2 to 8 |
| Surface spécifique | 200 m²/g |
| Porosité | 200 Å |
| Type de colonne | Reversed Phase |
| Phase stationnaire | C30 |
MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 10 μm Particle Size
The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
| Phase | Reversed Phase |
|---|---|
| Matrice | C18 (octadecyl) Phase/silica particle platform |
| “End-capped” | Yes |
| Gamme de produits | Discovery |
| Surface spécifique | 100 m2/g |
| Granulométrie | 10 μm |
| Technique | Wide Pore HPLC |
| Porosité | 300 Å |
| Phase stationnaire | C18 (octadecyl) |
Thermo Scientific™ Accucore™ 150-C4 HPLC Columns
Use this specially designed solid core column with a C4 functionality for biomolecules that bind too tightly to C8 or C18 columns.
| À utiliser avec | HPLC |
|---|---|
| Phase | Reversed Phase |
| Température | 60°C |
| Taux de carbone | 2% |
| “End-capped” | Yes |
| Type d’USP | L26 |
| pH | 2 to 9 |
| Surface spécifique | 80 m²/g |
| Granulométrie | 2.6 μm |
| Porosité | 150 Å |
| Type de colonne | Reversed Phase |
| Matériau de remplissage | Spherical, Solid Core, Ultrapure Silica |
| Phase stationnaire | C4 |
Thermo Scientific™ Accucore™ Phenyl-Hexyl HPLC Columns
Use this solid core column for fast, high-resolution separations of aromatic and moderately polar compounds.
| Phase | Reversed Phase |
|---|---|
| Forme des particules | Spherical Solid Core |
| Type d’USP | L11 |
| Surface spécifique | 130 m²/g |
| Porosité | 80 Å |
| Matériau de remplissage | Spherical, Solid Core, Ultrapure Silica |
| Température | 70°C |
| Taux de carbone | 5% |
| “End-capped” | Yes |
| Gamme de produits | Accucore |
| pH | 2 to 8 |
| Granulométrie | 2.6 μm |
| Pression max. | 14500 psi (1000 bar) |
| Type de colonne | Reversed Phase |
Restek™ Pinnacle™ DB C8 Specialty/Application Specific HPLC Column, 5 μm
Base-deactivated spherical silica optimized for UHPLC stability.
| À utiliser avec | Analysis of Basic Compounds or Bases Mixed with Acids/Neutrals |
|---|---|
| Épaisseur du film | 5 μm |
| Phase | Reversed Phase |
| Forme des particules | Spherical |
| Matériau | Stainless Steel |
| Type d’USP | L7 |
| Porosité | 140 Å |
| Matériau de remplissage | Silica |
| Phase stationnaire | C8 |
| Température | 80°C |
| Type de ligand | Monomeric C8 |
| Taux de carbone | 6% |
| “End-capped” | Yes |
| pH | 2.5 to 10 |
| Quantité | 5 g |
| Granulométrie | 5 μm |
| Technique | UHPLC |
| Pression max. | 4000 psi (275 bar) |
| Type de colonne | Specialty/Application Specific |
Restek™ Viva™ HPLC Columns with C18 Column Packing, 5μm Particle Size
Wide bore silica columns excellent for general-purpose applications and separating peptides or proteins
| À utiliser avec | Analyzing Large Molecules and Biomolecules |
|---|---|
| Épaisseur du film | 5 μm |
| Phase | Reversed Phase |
| Forme des particules | Spherical |
| Type d’USP | L1 |
| Porosité | 300 Å |
| Matériau de remplissage | Silica |
| Phase stationnaire | C18 |
| Type | Column |
| Température | 80°C |
| Taux de carbone | 9% |
| “End-capped” | Yes |
| pH | 2.5 to 10 |
| Granulométrie | 5 μm |
| Type de colonne | Reversed Phase |
| Type | Cartridge |
|---|---|
| Phase | Reversed Phase |
| Matériau | Stainless Steel |
| “End-capped” | Yes |
| Type d’USP | L1 |
| Porosité | 100 Å |
| Type de colonne | Reversed Phase |
| Matériau de remplissage | Silica |
| Phase stationnaire | C18 |
Hamilton™ PRP™-3 Reversed Phase HPLC Columns
PRP-3 polymeric 300 Å columns for gradient separations of peptides and proteins
| Phase | Reversed Phase |
|---|---|
| Température | 5°C to 85°C |
| Type d’USP | L21 |
| pH | 1 to 13 |
| Granulométrie | 10 μm |
| Porosité | 300 Å |
| Pression max. | 5000 psi (345 bar) |
| Type de colonne | Reversed Phase |
| Matériau de remplissage | PSDVB (Polystyrene Divinylbenzene) |
| Phase stationnaire | PRP-3 |
Hamilton™ PRP-1 Reversed Phase HPLC Columns, 10um Particle Size: Stainless Steel
Stable alternative to C8 or C18 Columns
| Compatibilité de phase mobile | pH 1 to 13, 0 to 100% aqueous, organic modifier |
|---|---|
| Type | Column |
| Phase | Reversed Phase |
| Température | 5°C to 85°C |
| Matériau | Stainless Steel |
| Type d’USP | L21 |
| pH | 1 to 13 |
| Porosité | 100 Å |
| Pression max. | 5000 psi (345 bar) |
| Type de colonne | Reversed Phase |
| Matériau de remplissage | PSDVB (Polystyrene Divinylbenzene) |
| Phase stationnaire | PRP-1 |
| Format de colonne | Analytical Column |
Hamilton™ PRP-1 Reversed Phase HPLC Columns, 10um Particle Size: PEEK
Stable alternative to C8 or C18 Columns
| Débit | 1.0 mL/min |
|---|---|
| Phase | Reversed Phase |
| Matériau | PEEK |
| Type d’USP | L21 |
| Porosité | 100 Å |
| Diamètre (métrique) | 4.6 mm |
| Matériau de remplissage | PSDVB (Polystyrene Divinylbenzene) |
| Phase stationnaire | PRP-1 |
| Compatibilité de phase mobile | pH 1 to 13, 0 to 100% aqueous, organic modifier |
| Type | Column |
| Température | 5°C to 85°C |
| pH | 1 to 13 |
| Granulométrie | 10 μm |
| Pression max. | 5000 psi (345 bar) |
| Type de colonne | Reversed Phase |
| Format de colonne | Analytical Column |