Flow Cytometry Compensation, Counting and Calibration

Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer

A buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. Designed for use in immunofluorescent staining protocols of cells in suspension.

Invitrogen™ eBioscience™ Fixation/Permeabilization Concentrate

Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

Invitrogen™ OneComp eBeads™ Compensation Beads

Spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls. OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test

Invitrogen™ UltraComp eBeads™ Spectral Unmixing Beads

UltraComp eBeads Spectral Unmixing Beads enhance spectral flow cytometry by providing precise single-color spectral unmixing control. Compatible with various antibodies and laser excitations, these third-generation beads offer optimized fluorophore unmixing, ensuring accurate results and superior performance.

Invitrogen™ Lysing Solution, Whole Blood Lysing Solution  

Lysing Solution, Whole Blood Lysing Solution

Invitrogen™ eBioscience™ Fixation/Permeabilization Diluent

Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

Micro Particle Size Standard, Based on Polystyrene Monodisperse, Analytical Standard, Size: 0.2 m, MilliporeSigma™ Supelco™

Invitrogen™ Cell Sorting Set-up Beads (for blue lasers)  

Approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow.

Invitrogen™ eBioscience™ Human Regulatory T Cell Staining Kit #2

Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.

Invitrogen™ eBioscience™ Human Th17 Cytokine Staining Panel

Includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage (IL-17A, IL-17F, IL-21 and IL-22).

Invitrogen™ eBioscience™ Human Regulatory T Cell Staining Kit #3

Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.

Invitrogen™ eBioscience™ Human Regulatory T Cell Whole Blood Staining Kit

Contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3+ cells in whole blood samples.

Molecular Probes™ Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 2.5 μm  

Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 2.5 μm

Micro Particle Size Standard, Based on Polystyrene Monodisperse, Analytical Standard, Size: 100 m, MilliporeSigma™ Supelco™

Invitrogen™ CountBright™ Plus Ready Tubes

CountBright Plus Ready Tubes offer the same advantages as CountBright Plus Absolute Counting Beads, but in ready-to-use tubes. This not only improves the accuracy and repeatability of cell counting, but also allows for convenient room temperature storage for up to 12 months.