Flow Cytometry Compensation, Counting and Calibration
Flow Cytometry Compensation, Counting and Calibration
Invitrogen™ eBioscience™ Mouse Hematopoietic Lineage Biotin Panel
Contains 5 biotinylated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their cell surface antigen expression.
Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A)
Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.
Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set
Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines
Invitrogen™ OneComp eBeads™ Compensation Beads
Spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls. OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test
Invitrogen™ UltraComp eBeads™ Compensation Beads
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test.
Invitrogen™ 123count eBeads™ Counting Beads
Intended for use in absolute counting of cells or other particles by flow cytometry. 123count eBeads are 7μm microparticles containing encapsulated dyes compatible with blue (488nm) and violet (405nm) excitation sources and emitting fluorescence between approximately 500nm and 750nm.
Invitrogen™ eBioscience™ Human Regulatory T Cell Staining Kit
Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.
Invitrogen™ eBioscience™ IC Fixation Buffer
Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.
Invitrogen™ eBioscience™ Fixation/Permeabilization Diluent
Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent
For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.
Invitrogen™ eBioscience™ Permeabilization Buffer (10X)
Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.
Invitrogen™ eBioscience™ Fixation/Permeabilization Concentrate
Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
Invitrogen™ eBioscience™ Mouse Regulatory T Cell Staining Kit #2
Formulated & optimized for the staining of mouse splenocytes with the FJK-16s monoclonal antibody. eBioscience™ Mouse Regulatory T Cell Staining Kit #2 includes: Anti-Mouse CD4 FITC, CD25 PE and CD16/32, Anti-Mouse/Rat Foxp3 APC, Rat IgG2a Isotype Control APC, staining buffer, fixation concentrate.
Invitrogen™ eBioscience™ Mouse Regulatory T Cell Staining Kit #1
Formulated and optimized for the staining of mouse splenocytes with the FJK-16s monoclonal antibody. eBioscience™ Mouse Regulatory T Cell Staining Kit #1 includes: Anti-Mouse CD4 FITC, CD25 APC and CD16/32, Anti-Mouse/Rat Foxp3 PE, Rat IgG2a Isotype Control PE, staining buffer, fixation concentrate.
Invitrogen™ eBioscience™ Mouse Cytokine Positive Control Cells
Recommended for use as positive control cells when establishing an intracellular cytokine staining protocol.
Invitrogen™ eBioscience™ Essential Human Th1/Th17 Phenotyping Kit
Invitrogen™ eBioscience™ Essential Human Th1/Th17 Phenotyping Kit is designed for flow cytometry-based identity testing of Type 1 and Type 17 T-helper cells
Invitrogen™ eBioscience™ Essential Human Treg Phenotyping Kit
Invitrogen™ eBioscience™ Essential Human Treg Phenotyping Kit is designed for flow cytometry-based identity testing of regulatory T-cells (Tregs).
Invitrogen™ eBioscience™ Essential Human T-Cell Phenotyping Kit
Invitrogen™ eBioscience™ Essential Human T-Cell Phenotyping Kit is designed for flow cytometry-based identity testing of T-cells.
Invitrogen™ eBioscience™ Essential Human Treg Phenotyping Panel & Staining Buffers
Invitrogen™ eBioscience™ Essential Human Treg Phenotyping Panel and Staining Buffers are designed for flow cytometry-based identity testing of regulatory T-cells (Tregs).
Invitrogen™ eBioscience™ Essential Human T-Cell Phenotyping Panel
Invitrogen™ eBioscience™ Essential Human T-Cell Phenotyping Panel is designed for flow cytometry-based identity testing of T-cells
Invitrogen™ eBioscience™ Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)
Can be used for induction and subsequent intracellular detection of cytokines and other secreted proteins in both in vitro-cultured and ex vivo cells
Invitrogen™ eBioscience™ Cell Stimulation Cocktail (500X)
Cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.
Micro Particles Based on Melamine Resin, Carboxylate-Modified, FITC-marked, Analytical Standard, Size: 1 m, MilliporeSigma™ Supelco™
Fluorescent marker for macrostructures, e.g. microtubules; Aldehyde modification for coupling with amines
Thermo Scientific™ 8000 Series Silica Particle Size Standards
Provide greater durability and chemical stability when using solvents with these monodisperse silica microspheres used in instrument calibration and as spacers.
Invitrogen™ eBioscience™ Mouse Regulatory T Cell Staining Kit #3
Formulated & optimized for the staining of mouse splenocytes with the FJK-16s monoclonal antibody. eBioscience™ Mouse Regulatory T Cell Staining Kit #3 includes: Anti-Mouse CD4 FITC, CD25 PE & CD16/32, Anti-Mouse/Rat Foxp3 PE-Cy5, Rat IgG2a Isotype Control PE-Cy5, stain buffer, fixation concentrate.
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