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DNA Cloning and Mapping and Mutagenesis

Chemical reagents, kits, and other products used for DNA cloning and mapping, and to carry out the process of mutagenesis.
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1

Thermo Scientific™ Lambda DNA (dam–, dcm–)

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

2

Thermo Scientific™ Lambda DNA

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

3

Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix  DFS Item

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Type de contrôle Positive Control
Type de produit HiFi Master Mix
Gamme de produits GENEART
Nombre de fragments Up to 6 fragments
Étape du flux de travail DNA Assembly
Conditions d’expédition Ambient, Wet Ice, Dry Ice
Méthode de clonage Seamless cloning
À utiliser avec (application) Seamless Cloning & Genetic Assembly
Contenu et stockage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
4

Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells  DFS Item

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

5

Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, chemically competent cells  DFS Item

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

6

Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, electrocompetent cells  DFS Item

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

7

Invitrogen™ GeneArt™ Seamless PLUS Cloning and Assembly Kit  DFS Item

The complete kit for simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute or less, room temperature reaction

8

Invitrogen™ TrueTag™ Donor DNA Kit, 6xHis  DFS Item

The Invitrogen TrueTag Donor DNA Kit, 6xHis, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

9

Thermo Scientific™ aLICator LIC Cloning and Expression Kit 1 (untagged)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

10

Thermo Scientific™ pUC19 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

11

Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

12

Thermo Scientific™ phiX174 RF1 DNA

Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.

13

Invitrogen™ TrueTag™ Donor DNA Kit, DDK  DFS Item

The Invitrogen TrueTag Donor DNA Kit, DDK, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

14

Invitrogen™ TrueTag™ Donor DNA Kit, HA  DFS Item

The Invitrogen TrueTag Donor DNA Kit, HA, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

15

Thermo Scientific™ aLICator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WQ)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.