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Thermo Scientific™ pUC18 DNA

Catalog No. FERSD0051
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50 μg
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FERSD0051 50 μg
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Catalog No. FERSD0051 Supplier Thermo Scientific™ Supplier No. SD0051
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Description

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

pUC18 and pUC19 vectors are small, high copy number, E. coli plasmids, 2686 bp in length. They are identical except that they contain multiple cloning sites (MCS) arranged in opposite orientations.

Highlights

  • Purified by chromatography using proprietary patented technology
  • More than 90% in the supercoiled form
  • Isolated from E.coli (dam+, dcm+)
  • For pUC18 DNA sequence, pUC19 DNA sequence, sequence analysis and map creation, see free online REviewer tool.

Specifications

Cloning Method Restriction Enzyme⁄ MCS
Concentration 0.5 μg/μL
Sample Type DNA
Final Product Type DNA
For Use With (Application) Cloning
Product Type DNA
Quantity 50 μg
Vector pUC18

Frequently Asked Questions (FAQs)

I'm getting no colonies after transformation. What should I do?

No colonies may occur due to the following problems:

Bacteria were not competent. Use the pUC18 vector included with the One Shot module to check the transformation efficiency of the cells.
- Incorrect concentration of antibiotic on plates, or the plates are too old. Use 100 µg/mL of ampicillin or 50 µg/mL kanamycin. Be sure ampicillin plates are fresh (< 1 month old).
- The product was phosphorylated (TOPO cloning only). Phosphorylated products can be TA-cloned but not TOPO-cloned. This is because the necessary phosphate group is contained within the topoisomerase-DNA intermediate complex of the vector. The TOPO vector has a 3' phosphate to which topoisomerase is covalently bound and a 5' phosphate. The non- TOPO vectors (TA and Blunt) have a 3' OH and a 5' phosphate. Phosphorylated products should be phosphatased (CIP) before TOPO-cloning.

For Research Use Only. Not for use in diagnostic procedures.