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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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Cytiva HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

EDGE
Content And Storage Store frozen
Sterilization Method 0.1 μm Sterile-filtered
Form Liquid
Shelf Life 24 Months
Source Porcine pancreas
2

Corning™ 0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

EDGE
3

Corning™ Accutase™ Cell Detachment Solution, Liquid

Efficient, with gentle detachment of adherent cells within minutes

EDGE
Content And Storage Store at -20°C.
Form Liquid
Formulation 1 X Accutase enzymes prepared in Dulbeccos PBS (0.2g/L KCl, 0.2g/L KH2PO5, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5 mm EDTA·4Na and 3 mg/L Phenol Red.
Sterility Sterile
4

Gibco™ Trypsin-EDTA (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Chelators EDTA
Content And Storage Storage conditions: -5°C to -20°C
Shipping conditions: Wet ice
Shelf life: 24 months from date of manufacture
Form Liquid
Tested For In vitro Bioassay
Osmolality 270 - 320 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco
5

Gibco™ TrypLE™ Select Enzyme (1X), no phenol red  DFS Item

Gibco™ TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells.

Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No Phenol Red
Shelf Life 24 Months
Concentration 1 X
6

Gibco™ Trypsin (2.5%), no phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

7

Gibco™ Versene Solution  DFS Item
GREENER_CHOICE

An EDTA solution for use as a gentle non-enzymatic cell dissociation reagent

8

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation.

10

Gibco™ Trypsin-EDTA (0.5%), no phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

13

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium, Magnesium or Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Content And Storage -20°C
Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No Calcium,No Magnesium,No Sodium Bicarbonate
pH 7 to 7.6
Sterility Sterile
14

Invitrogen™ Accumax - Cell Aggregate Dissociation Medium

Dissociate cell clumps for cell counting, viral transfection assays, cell sorting, and flow cytometry as well as bioreactor scale-up.

15

Gibco™ Trypsin (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Shipping Condition Wet Ice
Chelators No EDTA
Content And Storage Storage conditions: -5 to -20°C.
Shelf life: 24 months from date of manufacture.
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality 280 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
Sterilization Method Sterile-filtered
With Additives Phenol Red
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco