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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

MilliporeSigma™ Calbiochem™ Trypsin Porcine MS Grade

A chemically modified trypsin that is resistant to autolysis and degradation

2

Gibco™ Soybean Trypsin Inhibitor, powder  DFS Item

Trypsin inhibited at a molar ratio of 1:1 and chymotrypsin and plasmin to lesser extent

3

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells.

4

Corning™ Accutase™ Cell Detachment Solution, Liquid

Efficient, with gentle detachment of adherent cells within minutes

Stérilité Sterile
Formule 1 X Accutase enzymes prepared in Dulbeccos PBS (0.2g/L KCl, 0.2g/L KH2PO5, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5 mm EDTA·4Na and 3 mg/L Phenol Red.
Forme Liquid
Contenu et stockage Store at -20°C.
5

Invitrogen™ Accutase - Enzyme Cell Detachment Medium

Invitrogen™ Accutase Enzyme Cell Detachment Medium performs exceptionally well in detaching cells for analysis of cell surface markers and virus growth assay.

7

Gibco™ Collagenase, Type I, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

Type de produit Collagenase
Stérilité Non-sterile
Durée de conservation 24 Months
Conditions d’expédition Room Temperature
Forme Lyophilized
Contenu et stockage Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
8

Gibco™ Collagenase, Type II, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen

9

Gibco™ Collagenase, Type IV, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen

10

MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x)

Used to dissociate primary cells, tissues, and tumors with an increased plating efficiency. MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x) is PBS based formulation of chelating agents that contain agents to stabilize their activity on cells.

Type de produit Cell Culture Dissociation Reagent
Stérilité Sterile
Concentration 1 X
Formule Chelating and stabilizing agents
Durée de conservation 2 Years
À utiliser avec (application) Cell Dissociation
Forme Liquid
Contenu et stockage Store at room temperature or at 4°C.
11

Corning™ 10% SDS (Sodium Dodecyl Sulfate)

Powerful protein denaturant produced under highest industry standards

12

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium, Magnesium or Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Type de produit Cell Culture Dissociation Reagent
Stérilité Sterile
pH 7 to 7.6
Sans additif No Calcium,No Magnesium,No Sodium Bicarbonate
Forme Liquid
Contenu et stockage -20°C
13

MilliporeSigma™ Xeno-Free 0.05% Trypsin/0.02% EDTA Kit

Useful for routine detachment of cells from standard tissue plasticware and adhesion coated plasticware under animal-free conditions..

14

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

15

MilliporeSigma™ ESGRO Complete™ Accutase™ Cell Detachment Solution

a cell detachment solution of proteolytic and collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.