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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 927 results
1

Thermo Scientific™ Hypersil™ Phenyl-BDS HPLC Columns

Use as a robust, general-purpose column with aromatic selectivity for QC/QA labs.

Pore Size 130 Å
Carbon Load 5%
Stationary Phase Phenyl (Ph)
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Base-Deactivated Silica
Column Type Reversed Phase
Surface Area 170 m²/g
Max. Pressure 5800 psi (400 bar)
USP Type L11
2

TSKgel™ Phenyl-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

The most hydrophobic among the three TSKgel™ HIC columns, TSKgel™ Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules.

3

MilliporeSigma™ Supelco™ Ascentis™ Express 90 Å PAH, 2.7 μm HPLC Guard Cartridge

The unendcapped trifunctional C18 stationary phase of Ascentis™ Express PAH provides a stable, reversed-phase packing that can be used for analysis of acids, bases, and zwitterionic compounds, but has been specifically designed for the separation of polycyclic aromatic hydrocarbons.

Pore Size 90 Å
Particle Size 2.7 μm
Temperature <60°C
Matrix Fused-Core™ Silica Particle Platform Particle Platform Superficially Porous Particle
Length (Metric) 0.5 cm
Column Type Reversed Phase
pH 2 to 9
Quantity 3/Pk.
Material Stainless Steel
Surface Area 135 m2/g
Max. Pressure 600 bar
5

MilliporeSigma™ Supelco™ Proteomix™ SAX-NP5 Strong Anion Exchange Column, 5 μm Particle Size, PEEK

Used for the analyis or small scale purification of negatively charged biopolymers, such as glycoprotein isoforms, pegylated proteins and oligonucleotide.

Pore Size Nonporous
Particle Size 5 μm
Stationary Phase Ammonium Ion, Quaternary
Phase Anion Exchange
Matrix Spherical PS-DVB/Ammonium Ion, Quaternary Phase
pH 2.0 to 12.0
Material PEEK
Technique Anion Exchange HPLC
Product Line Proteomix
6

Thermo Scientific™ Acclaim™ Mixed Mode WAX-1 HPLC Columns
GREENER_CHOICE

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific™ Acclaim™ Mixed-Mode WAX-1 LC columns.

Pore Size 120 Å
Phase Reversed Phase, Ion Exchange, HILIC
Column Type Mixed Mode
pH 2.5 to 7.5
Surface Area 300 m²/g
Product Line Acclaim
USP Type L78
7

MilliporeSigma™ Supelco™ Kromasil™ Eternity™ XT C18 HPLC Column, 100 Å, 1.8 μm Particle Size

Kromasil™ Eternity™ and Eternity™XT can be used in a wider pH window than regular silica-based HPLC materials, giving more flexibility than ever when running analytical applications or optimizing for large-scale applications.

Pore Size 100 Å
Particle Size 1.8 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase
Diameter (Metric) 2.1 mm
Product Line Kromasil Eternity
8

Thermo Scientific™ ProPac™ PA1 HPLC Columns

Efficiently analyze and purify hydrophilic anionic proteins and peptides with ProPac™ PA1 LC Columns.

Pore Size Nonporous
Particle Size 10 μm
Flow Rate 0.9 to 1.5 mL/min
Stationary Phase Alkyl Quaternary Amine
Phase Ion Exchange
For Use With BioLC Chromatography System
Packing Material Spherical, Nonporous Polymer
Column Type Ion Exchange
Quantity Each
Green Features FSC
Material PEEK
Max. Pressure 5000 psi (345 bar)
Product Line ProPac
9

MilliporeSigma™ Supelco™ Carbosieve™ SIII Thermal Desorption Tubes

Suitable for use with Air Monitoring

Type Desorption Tube
Matrix Carbosieve™ S-III Carbon Molecular Sieve
For Use With (Equipment) PerkinElmer, Markes, DANI, OI Analytical, and Shimadzu Instruments
Diameter (English) Outer 1/4 in.
Packaging Quantity 10/Pk.
Material Stainless Steel
Overall Length (English) 3.5 in.
For Use With (Application) Air Monitoring
Methods EPA TO-2
Product Line Carbosieve
10

Thermo Scientific™ POROS™ Heparin 50 μm Affinity Columns
GREENER_CHOICE

Thermo Scientific™ POROS™ Prepacked Heparin, Affinity Column. POROS Heparin is based on an immobilized Heparin functional group designed for high throughput purification of proteins or viruses with specific Heparin affinity.

Particle Size 50 μm
Stationary Phase Heparin (Porcine)
Column Type Affinity
Quantity 1 Column
Material PEEK
Max. Pressure 750 psi (50 bar)
Product Line POROS
11

Thermo Scientific™ Acclaim™ Explosives E2 HPLC Columns
GREENER_CHOICE

Use for baseline resolution of all 14 explosives listed in EPA Method 531.2.

Pore Size 120 Å
Stationary Phase Proprietary
Temperature 50°C
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Column Type Specialty/Application Specific
pH 2.5 to 7.5
Surface Area 300 m²/g
Product Line Acclaim
12

TSKgel™ Amide-80 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products.

Pore Size 100 Å
Particle Size 3 μm
Stationary Phase Carbamoyl
Temperature 10°C to 50°C
Max. Pressure 200 bar
Product Line TSKgel
USP Type L68
13

MilliporeSigma™ Supelco™ SRT™-C SEC-300 Gel Filtration Column, 5 μm Particle Size, Stainless Steel

The bonded phase in SRT™-C SEC columns is more polar than the hydrophilic phase used in SRT™ columns, making SRT™-C columns better suited for the gel filtration analysis of more hydrophobic bioopolymers such as membrane proteins and pegylated antibodies.

Pore Size 300 Å
Particle Size 5 μm
Stationary Phase Hydrophilic Polymer
Phase Size Exclusion
Matrix Hydrophilic Polymer Phase/Spherical Silica
Length (Metric) 30 cm
pH 2.0 to 8.5
Material Stainless Steel
Technique SEC HPLC
Product Line SRT
14

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns
GREENER_CHOICE

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Pore Size 120 Å
Carbon Load 8%
Stationary Phase Proprietary
Temperature 70°C
Phase HILIC
For Use With LC/MS
Endcapped Yes
Column Type HILIC
pH 2 to 8
Surface Area 300 m²/g
Product Line Acclaim
15

MilliporeSigma™ Supelco™ Unix™ SEC-300 UHPLC Column, 1.8 μm Particle Size

Provides high stability and negligible non-specific interactions.

Pore Size 300 Å
Particle Size 1.8 μm
Stationary Phase Neutral, Hydrophilic Film Bonded to Silica
Phase Size Exclusion
Matrix Neutral, Hydrophilic Film Bonded to Silica Phase/Spherical Silica
Diameter (Metric) 4.6 mm
Technique SEC UHPLC
Product Line Unix