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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 928 results
1

MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

2

Thermo Scientific™ ProPac™ HIC-10 HPLC Columns
GREENER_CHOICE

Achieve high-resolution separation of proteins and peptides under nondenaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns.

Pore Size 300 Å
Particle Size 5 μm
Stationary Phase Amide/Ethyl
Temperature 40°C
Phase Hydrophobic Interaction
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Hydrophobic Interaction
pH 2.5 to 7.5
Material Stainless Steel
Surface Area 100 m²/g
Max. Pressure 3500 psi (240 bar)
3

Thermo Scientific™ BetaBasic™ Phenyl Columns, 5μm Particle Size

BetaBasic columns are highly efficient and reproducible for small molecules, peptides and protein digests

Pore Size 150 Å
Particle Size 5 μm
Carbon Load 7%
Stationary Phase Phenyl (Ph)
Phase Reversed Phase
For Use With LC/MS Separation
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
Surface Area 200 m²/g
Particle Shape Spherical
Film Thickness 5 μm
USP Type L11
4

Thermo Scientific™ Accucore™ XL C18 HPLC Columns

Use these solid core columns for optimal retention of a broad range of nonpolar analytes across multiple applications.

Pore Size 80 Å
Particle Size 4 μm
Carbon Load 7%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
pH 1 to 11
Surface Area 90 m²/g
Max. Pressure 8700 psi (600 bar)
Product Line Accucore
USP Type L1
5

Thermo Scientific™ BetaSil™ Phenyl/Hexyl Columns--5μm Particle Size

Offer unique selectivity

Pore Size 100 Å
Particle Size 5 μm
Carbon Load 11%
Stationary Phase Phenyl/Hexyl
Phase Reversed Phase
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 9
Product Line Beta
USP Type L11
6

MilliporeSigma™ Supelco™ Astec™ CYCLOBOND™ I 2000 HP-RSP Chiral HPLC Column, 5 μm Particle Size

Most beneficial for basic and neutral compounds.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase Cyclodextrin, Beta-
Phase Chiral
Matrix Cyclodextrin, Beta- Phase/Silica Particle Platform
Diameter (Metric) 4.6 mm
Technique Chiral HPLC
7

Thermo Scientific™ Hypersil™ APS-2 Amino (NH2) Mixed Mode HPLC Column, 5 μm, 4 mm x 300 mm

Column Format: Analytical Column; Carbon Load: 1.90%; Pore Size: 120 Å; Column Phase: Reversed Phase, Normal Phase, Ion Exchange, HILIC

8

Thermo Scientific™ Hypersil™ APS-2 Amino HPLC Column

Analyze sugars with this versatile amino propyl phase of Thermo Scientific™ Hypersil™ APS-2 Amino LC Columns.

Pore Size 120 Å
Particle Size 10 μm
Carbon Load 2%
Stationary Phase Amino (NH2)
Phase Reversed Phase, Normal Phase, Ion Exchange, HILIC
Packing Material Silica
Column Type Mixed Mode
pH 2.5 to 8
Surface Area 170 m²/g
USP Type L8
9

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-Si HPLC Column, 3 μm Particle Size

A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent.

Pore Size 120 Å
Particle Size 3 μm
Stationary Phase Silica
Phase Reversed Phase
Matrix Silica Phase/Silica Gel, Spherical Particle Platform
Length (Metric) 15 cm
Surface Area 170 m2/g
Product Line SUPELCOSIL
10

Thermo Scientific™ ProPac™ IMAC-10 HPLC Columns
GREENER_CHOICE

Resolve target proteins using a single column in a high-resolution gradient run with ProPac IMAC-10 LC Columns.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Lysozyne Poly(IDA) Grafts
Temperature 60°C
Phase Affinity Separation
Packing Material Spherical, Nonporous Polymer
Column Type Separation of Metal-Binding Proteins
pH 2 to 12
Max. Pressure 3000 psi (207 bar)
11

MilliporeSigma™ Supelco™ Nucleosil™ CN HPLC Column, 5 μm Particle Size

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase Cyano
Phase Reversed Phase, Normal Phase, HILIC
Endcapped No
Surface Area 350 m2/g
Product Line Nucleosil
12

TSKgel™ Amide 80 HPLC Column, 2 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ Amide-80 columns are used for the analysis of saccharides, glycans, oligosaccharides, peptides and polar compounds from natural products.

Particle Size 2 μm
Stationary Phase Carbamoyl
Temperature 50°C
Phase HILIC
Matrix Spherical Silica
pH 2.0 - 7.5
Material Stainless Steel Hardware
Surface Area 450 m2/g
Max. Pressure 150 bar
Product Line TSKgel
USP Type L68
13

MilliporeSigma™ Supelco™ Ascentis™ Express RP-Amide UHPLC Column, 2 μm Particle Size

The one-step bonded, end-capped, amide-based polar-embedded stationary phase of Ascentis™ Express 2 μm RP-Amide provides, a stable, reversed phase packing with decreased hydrophobic character. Ascentis™ Express 2 μm RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8.

Pore Size 90 Å
Particle Size 2 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Fused-Core Particle Platform
Endcapped Yes
Technique Fused-Core UHPLC
Product Line Ascentis
14

TSKgel™ SuperQ-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel SuperQ-5PW Columns are packed with porous hydrophilic polymer beads which are modified with a strong anion exchanger. Columns are used for the separation and analysis of proteins, oligonucleotides, and other biologically active molecules.

Particle Size 10 μm
Solvents ≤ 20% Organic Solvent
Stationary Phase Ammonium Ion (Quaternary Phase)
Temperature ≤45°C
Length (Metric) 7.5 cm
Ion Exchange Group Cl-
pH 2 to 12
Technique Anion Exchange
Max. Pressure 200 bar
Product Line TSKgel
USP Type L23
15

TSKgel™ Alpha-4000 HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Packed with rigid, porous polymer beads which show minimal shrinkage or swelling in different solvent systems. Columns are best used for the separation of polymers that are soluble in polar organic solvents.