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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 931 results
1

MilliporeSigma™ Supelco™ Ascentis™ RP-Amide HPLC Column, 3 μm Particle Size

Ascentis™ RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis™ RP-Amide is the first choice in embedded polar group HPLC phases.

Pore Size 100 Å
Particle Size 3 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 8
Technique Fused-Core HPLC
Surface Area 450 m2/g
Product Line Ascentis
2

Thermo Scientific™ Hypersil GOLD™ C8 HPLC Columns

Have analytes with medium hydrophobicity or need a less hydrophobic phase to obtain optimum retention? These columns provide outstanding sensitivity and excellent peak shape.

Pore Size 175 Å
Carbon Load 8%
Stationary Phase C8
Temperature 60°C
pH 2 to 9
Surface Area 220 m²/g
USP Type L7
3

Thermo Scientific™ ProPac™ HIC-10 HPLC Columns
GREENER_CHOICE

Achieve high-resolution separation of proteins and peptides under nondenaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns.

Pore Size 300 Å
Particle Size 5 μm
Stationary Phase Amide/Ethyl
Temperature 40°C
Phase Hydrophobic Interaction
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Hydrophobic Interaction
pH 2.5 to 7.5
Material Stainless Steel
Surface Area 100 m²/g
Max. Pressure 3500 psi (240 bar)
4

TSKgel™ UP-SW3000 HPLC Column, 2 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ UP-SW3000 columns deliver dramatically increased resolution between fragments, monomers, and aggregates.

Pore Size 250 Å
Particle Size 2 μm
Solvents 0 to 30% aqueous soluble organic solvents
Flow Rate 0.35 mL/min.
Stationary Phase Diol
Temperature 10°C to 30°C
Phase Size Exclusion
Matrix Silica Particle Platform
Diameter (Metric) 4.6 mm
pH 2.5 - 7.5
Max. Pressure 340 bar
Product Line TSKgel
USP Type L20, L59
5

TSKgel™ Top-Off, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

For use with TSKgel™ PWxl and G-DNA-PW Columns, 10 μm. Shipped in water.

6

MilliporeSigma™ Supelco™ Proteomix™ WAX-NP1.7 Weak Anion Exchange Column, 1.7 μm Particle Size, Stainless Steel

Used for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.

Pore Size Nonporous
Particle Size 1.7 μm
Stationary Phase Weak Anion Exchange
Phase Anion Exchange
Matrix Spherical PS-DVB/Weak Anion Exchange Phase
pH 2.0 to 12.0
Material Stainless Steel
Technique Anion Exchange HPLC
Product Line Proteomix
7

MilliporeSigma™ Supelco™ Omnifit™ EZ Benchmark Columns with 2 Fixed Endpieces

Omnifit™ Labware complete columns are supplied assembled and ready for use, with tubing and fittings to connect the column into an existing system.

Column Type Chromatography
Product Line Omnifit
8

MilliporeSigma™ Supelco™ Ascentis™ Express ES-Cyano HPLC Column, 2.7 μm Particle Size

The sterically protected, extensively endcapped diisopropyl-cyanopropylsilane stationary phase of Ascentis™ Express ES-Cyano provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Diisopropyl-Cyanopropylsilane
Phase Reversed Phase
Matrix Diisopropyl-Cyanopropylsilane Phase/Fused-Core Particle Platform
Endcapped Yes
Technique Fused-Core HPLC
Product Line Ascentis
9

Thermo Scientific™ Hypersil GOLD™ Silica HPLC Columns

Efficiently separate nonpolar and moderately polar organic compounds by normal phase chromatography using Thermo Scientific™ Hypersil GOLD™ Silica LC Columns.

Pore Size 175 Å
Stationary Phase Silica
Temperature 60°C
Phase Normal Phase and HILIC
pH 2 to 8
Surface Area 220 m²/g
USP Type L3
10

MilliporeSigma™ Supelco™ Zenix™ SEC-100 Gel Filtration HPLC Column, 3 μm Particle Size, PEEK

Like their 5 micron siblings, 3 micron Zenix™ SEC columns feature a hydrophilic surface layer to prevent the sample from interacting with the underlying silica.

11

Thermo Scientific™ Acclaim™ Polar Advantage HPLC Columns
GREENER_CHOICE

Use for reversed-phase separation of basic or pharmaceutical compounds using highly aqueous mobile phases.

Pore Size 120 Å
Carbon Load 17%
Stationary Phase Polar Embedded
Temperature 60°C
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 300 m²/g
USP Type L60
12

Thermo Scientific™ Syncronis™ C18 Columns

Use for hydrophobic interaction reversed-phase separations.

Pore Size 100 Å
Carbon Load 16%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 9
Surface Area 320 m²/g
USP Type L1
13

MilliporeSigma™ Supelco™ Kromasil C8 HPLC Column, 100 Å, 10 μm Particle SIze

For small molecules and peptides.

Pore Size 100 Å
Particle Size 10 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase
Product Line Kromasil
14

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-18-DB HPLC Column, 5 μm Particle Size

SUPELCOSIL™ LC-DB phases are specially deactivated for basic compounds.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase C18 (Octadecyl)
Phase Reversed Phase
Matrix C18 (Octadecyl) Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
15

MilliporeSigma™ Supelco™ BIOshell™ Glycan HPLC Columns, 2.7 μm Particle Size

BIOshell™ Glycan HPLC Columns are specifically engineered to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Hydroxy, Penta-, Spherical Silica Particle Platform
Phase Reversed Phase
Matrix Hydroxy, Penta- Phase, Spherical Silica Particle Platform
Endcapped No
pH 2 to 9
Material Stainless Steel
Technique Fused-Core HPLC
Surface Area 135 m2/g
Product Line BIOshell