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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1

TSKgel™ G4000SW HPLC Column, 17 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G4000SW SEC columns provide excellent separation of proteins and other biomolecules.

Pore Size 450 Å
Particle Size 17 μm
Solvents 0 to 100% aqueous soluble organic solvents
Flow Rate 8 mL/min.
Stationary Phase Diol
Temperature 10°C to 30°C
Phase Size Exclusion
Matrix Silica Particle Platform
Diameter (Metric) 21.5 mm
pH 2.5 - 7.5
Material Stainless Steel Hardware
Product Line TSKgel
USP Type L20, L33, L59
2

TSKgel™ Octadecyl-NPR HPLC Column, 2.5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

The highly cross-linked, polymethacrylate base material of TSKgel Octadecyl-NPR provides excellent stability in high pH buffer systems. It can withstand rigorous cleaning with either acid or base.

3

TSKgel™ SuperH4000 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Packed with rigid, porous polymer beads and used for the non-aqueous separation of polymers with molecular weights less than 400,000 Da.

4

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-PAH HPLC Column, 5 μm Particle Size

SUPELCOSIL™ LC-PAH columns were designed specifically for analyses of the priority pollutant PAHs listed in US EPA Method 610.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase C18 (Octadecyl)
Phase Reversed Phase
Matrix C18 (Octadecyl) Phase/Silica Gel, Spherical Particle Platform
Product Line SUPELCOSIL
5

MilliporeSigma™ Supelco™ TSKgel™ GMHHR-H(30) HT2 HPLC Column, 30 μm Particle Size, Stainless Steel

TSKgel™ Ultra-High Temperature columns are for the analysis of organic-soluble polymers at extremely elevated temperatures.

6

MilliporeSigma™ Supelco™ Ascentis™ Express C18 PCP HPLC Columns, 2.7 μm Particle Size

Ascentis™ Express C18 PCP columns have bonded phase that is pre-conditioned with phosphoric acid.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped Yes
Diameter (Metric) 4.6 mm
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
7

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-18-T HPLC Column, 5 μm Particle Size

SUPELCOSIL™ LC-18-T columns feature an octadecylsilane bonded phase and a special surface treatment for efficient separations of nucleotides.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase C18 (Octadecyl)
Phase Reversed Phase
Matrix C18 (Octadecyl) Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Diameter (Metric) 4.6 mm
Surface Area 170 m2/g
Product Line SUPELCOSIL
8

MilliporeSigma™ Supelco™ Proteomix™ WAX-NP10 Weak Anion Exchange Column, 10 μm Particle Size, Stainless Steel

Used for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Weak Anion Exchange
Phase Anion Exchange
Matrix Spherical PS-DVB/Weak Anion Exchange Phase
pH 2.0 to 12.0
Material Stainless Steel
Technique Anion Exchange HPLC
Product Line Proteomix
9

MilliporeSigma™ Supelco™ Proteomix™ WCX-NP10 Weak Cation Exchange Column, 10 μm Particle Size, Stainless Steel

Used for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Weak Cation Exchange
Phase Cation Exchange
Matrix Spherical PS-DVB/Weak Cation Exchange Phase
pH 2.0 to 12.0
Material Stainless Steel
Technique Cation Exchange
Product Line Proteomix
10

MilliporeSigma™ Supelco™ PTFE Fitting, Jacketed Liquid Chromatography Column, 30 cm

Constructed of borosilicate glass and are rated at a maximum pressure of 15 psi (1 bar)

Length (Metric) 30 cm
Column Type Chromatography
Material Borosilicate Glass (Column), PTFE (Fittings), Acrylic (Jacket)
11

Thermo Scientific™ SurePac™ Bio 550 SEC MDi™ HPLC Columns

Experience the future of Size-Exclusion Chromatography (SEC) with SurePac™ Bio 550 SEC MDi™ analytical columns and guards exclusively designed to deliver robust and reproducible performance at high resolution and throughput to accelerate your therapeutics analysis process.

Particle Size 3 μm
Stationary Phase Proprietary
Temperature 20°C to 60°C
Phase Size Exclusion
Packing Material Monodisperse Silica Resin
pH 2 to 8
Quantity 1 Ea.
Technique SEC
12

Thermo Scientific™ HyperREZ™ XP Carbohydrate Pb2+ HPLC Columns

HyperREZ XP Carbohydrate Pb2+ LC Columns are used for the appropriate counter-ion to meet application requirements.

Particle Size 8 μm
Stationary Phase Pb2+ Counter Ion
Temperature 90°C
Phase Ligand Exchange
For Use With LC/MS
Packing Material Spherical, Microporous Polymer
pH 1 to 11
Max. Pressure 725 psi (50 bar)
USP Type L34
13

Thermo Scientific™ POROS™ CaptureSelect™ GCSF Affinty HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ GCSF HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands.

Pore Size 2000 Å
Stationary Phase Granulocyte ColonyStimulating Factor affinity ligand, human-specific
Column Type Affinity
Elution Volume Max 29 column volumes
Quantity 1 Column
Material PEEK
For Use With (Application) FPLC
High-throughput Compatibility Automated Protocols
Max. Pressure 2600 psi, 180 bar
Particle Size 20 μm
Matrix POROS
Target Molecule Protein
Product Type Column
Binding Capacity 5.2 mg/mL (ligand density)
Purification Target Protein
Elution Volume Min 14 column volumes
Product Line CaptureSelect, POROS
14

TSKgel™ Alpha-3000 HPLC Column, 7 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Packed with rigid, porous polymer beads which show minimal shrinkage or swelling in different solvent systems. Columns are best used for the separation of polymers that are soluble in polar organic solvents.

15

MilliporeSigma™ Supelco™ Ascentis™ Express C18 Micron Capillary HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower back pressures.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis