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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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Keyword Search:
1 – 15 of 933 results
1

Thermo Scientific™ ProPac™ SCX-20 Strong Cation Exchange HPLC Column
GREENER_CHOICE

Meet today's demands in protein separations using this high resolution strong cation exchange (SCX) HPLC column.

Stationary Phase Sulfonate
Temperature 60°C
Phase Ion Exchange
Column Type Ion Exchange
Quantity Each
Green Features FSC
Max. Pressure 3000 psi (207 bar)
Column Format Analytical Column
Particle Size 10 μm
Packing Material Ethylvinylbenzene Divinylbenzene
Diameter (Metric) 4 mm
pH 2 to 12
Technique Bio-LC
Product Line ProPac
2

TSKgel™ G1000HHR HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel G1000HHR Columns are packed with rigid, porous polymer beads. They are used for the non-aqueous separation of small molecules and oligomers less than 1,000 Da.

Shipping Condition Shipped in THF
Pore Size 15 Å
Particle Size 5 μm
Stationary Phase Polystyrene-divinylbenzene
Matrix Polystyrene-divinylbenzene Phase/Polymer Particle Platform
Length (Metric) 30 cm
Diameter (Metric) 7.8 mm
Technique SEC
Max. Pressure 50 bar
3

Thermo Scientific™ Acclaim™ Mixed Mode WAX-1 HPLC Columns
GREENER_CHOICE

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific™ Acclaim™ Mixed-Mode WAX-1 LC columns.

Pore Size 120 Å
Phase Reversed Phase, Ion Exchange, HILIC
Column Type Mixed Mode
pH 2.5 to 7.5
Surface Area 300 m²/g
Product Line Acclaim
USP Type L78
4

Thermo Scientific™ Hypersil GOLD™ SAX HPLC Columns

Analyze smaller organic molecules, including nucleotides and organic acids, quickly and efficiently with Thermo Scientific™ Hypersil™ GOLD SAX LC Columns.

Pore Size 175 Å
Carbon Load 2.5%
Stationary Phase Quaternary Amine
Temperature 60°C
Phase Ion Exchange
Column Type Ion Exchange
pH 2 to 8
Surface Area 220 m²/g
USP Type L14
5

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-NH2 HPLC Column, 3 μm Particle Size

The amino column is most often employed for the separation of mono- and disaccharides.

Pore Size 120 Å
Particle Size 3 μm
Stationary Phase Amino (NH2)
Phase Reversed Phase
Matrix Amino (NH2) Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
6

Thermo Scientific™ ProSwift™ RP-4H HPLC Columns
GREENER_CHOICE

Run fast protein separations at high linear velocities with Thermo Scientific™ ProSwift™ RP-4H LC Columns.

Pore Size Monolith
Solvents Most common Organic Solvents
Stationary Phase Phenyl (Ph)
Temperature 70°C
Phase Reversed Phase
For Use With LC/MS
Column Type Reversed Phase
Quantity Each
Green Features FSC
Column Chemistry Phenyl
Column Format Analytical Column
Particle Size Monolith
Type Analytical Column
Packing Material PSDVB (Polystyrene Divinylbenzene)
Diameter (Metric) 1 mm
pH 1 to 14
Technique Bio-LC
Product Line Monolith
7

MilliporeSigma™ Supelco™ LiChrospher™ RP-8 HPLC Column, 5 μm Particle Size

LiChrospher™ RP-8 HPLC Column is a traditionally produced spherical silica gel carrier with C-8 reversed-phase properties and is suitable for acidic, neutral and weakly basic compounds.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase
Endcapped No
Surface Area 350 m2/g
Product Line LiChrospher
8

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-18-DB HPLC Column, 3 μm Particle Size

SUPELCOSIL™ LC-DB phases are specially deactivated for basic compounds.

Pore Size 120 Å
Particle Size 3 μm
Stationary Phase C18 (Octadecyl)
Phase Reversed Phase
Matrix C18 (Octadecyl) Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
9

MilliporeSigma™ Supelco™ ChromegaChiral™ Chiral CCO F4 HPLC Column, 3 μm Particle Size

ChromegaChiral™ CCO F4 is a 4-Fluoro 3-methylphenyl cellulose phase which can be used in SFC or HPLC.

Pore Size 1000 Å
Particle Size 3 μm
Stationary Phase 2-fluoro 5-methylphenyl cellulose
Phase Chiral
Matrix 2-fluoro 5-methylphenyl cellulose Phase
Technique Chiral HPLC
Product Line ChromegaChiral
10

TSKgel™ ODS-80Ts HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

A general purpose column for low MW pharmaceuticals, bases, nucleosides, and nucleotides.

Shipping Condition Shipped in 70% Methanol, 30% Water
Particle Size 5 μm
Solvents 0-100% Organic Solvent
Carbon Load 15%
Stationary Phase C18 (octadecyl)
Temperature 10°C to 50°C
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Silica Particle Platform
Endcapped Yes
pH 2 to 7.5
Surface Area 425 m2/g
Product Line TSKgel
USP Type L1
11

TSKgel™ SuperHZ2000 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel SuperHZ2000 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 10,000 Da.

Pore Size 20 Å
Particle Size 3 μm
Stationary Phase Polystyrene-divinylbenzene
Temperature 60°C
Matrix Polystyrene-divinylbenzene Phase/Polymer Particle Platform
Length (Metric) 15 cm
Material Stainless Steel Hardware
Technique SEC
Max. Pressure 50 bar
Product Line TSKgel
12

Thermo Scientific™ Hypersil GOLD™ aQ C18 Polar Endcapped HPLC Columns

Retain and resolve polar analytes with highly aqueous mobile phases.

Pore Size 175 Å
Carbon Load 12%
Temperature 60°C
Phase Reversed Phase
Endcapped Polar
Column Type Reversed Phase
pH 2 to 9
Surface Area 220 m²/g
USP Type L1
13

MilliporeSigma™ Supelco™ Zenix™ SEC-150 Gel Filtration HPLC Column, 3 μm Particle Size, Stainless Steel

Like their 5 micron siblings, 3 micron Zenix™ SEC columns feature a hydrophilic surface layer to prevent the sample from interacting with the underlying silica.

Pore Size 150 Å
Particle Size 3 μm
Stationary Phase Hydrophilic Polymer
Phase Size Exclusion
Matrix Hydrophilic Polymer Phase/Spherical Silica
Length (Metric) 30 cm
pH 2.0 to 8.5
Material Stainless Steel
Technique SEC HPLC
Product Line Zenix
14

Thermo Scientific™ GlycanPac™ AXH-1 HPLC Columns
GREENER_CHOICE

Use this glycan analysis column for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules.

Phase HILIC and Ion Exchange
Column Type Mixed Mode
pH 2 to 8
Particle Shape Spherical silica
15

Thermo Scientific™ Single nanoViper™ and Double nanoViper™ PepMap™ Neo UHPLC Columns

Separate challenging peptide mapping samples with Thermo Scientific™ Double nanoViper™ PepMap™ Neo UHPLC columns.

Pore Size 100 Å
Particle Size 2 μm
Carbon Load 15%
Stationary Phase C18
Temperature 60°C
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 8
For Use With (Application) Peptide Mapping, LC-MS
Max. Pressure 22,000 psi (1500 bar)
USP Type L1