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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 931 results
1

Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
2

TSKgel™ SCX (H+) HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Packed with 5 μm porous, spherical particles which are surface modified with a strong (sulfonic acid) cation exchanger in the hydrogen. Column is optimized for the separation and analysis of organic acids, saccharides, and alcohols.

3

TSKgel™ Octadecyl-NPR HPLC Column, 2.5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

The highly cross-linked, polymethacrylate base material of TSKgel Octadecyl-NPR provides excellent stability in high pH buffer systems. It can withstand rigorous cleaning with either acid or base.

4

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-PAH HPLC Column, 5 μm Particle Size

SUPELCOSIL™ LC-PAH columns were designed specifically for analyses of the priority pollutant PAHs listed in US EPA Method 610.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase C18 (Octadecyl)
Phase Reversed Phase
Matrix C18 (Octadecyl) Phase/Silica Gel, Spherical Particle Platform
Product Line SUPELCOSIL
5

MilliporeSigma™ Supelco™ Carbopack X™ Thermal Desorption Tubes

Suitable for use with Air Monitoring

Type Thermal Desorption Tube
Matrix Carbopack™ x Graphitized Carbon Black (GCB)
For Use With (Equipment) PerkinElmer, Markes, DANI, OI Analytical, and Shimadzu Instruments
Diameter (English) Outer 1/4 in.
Packaging Quantity 10/Pk.
Material Glass
Overall Length (English) 3.5 in.
For Use With (Application) Air Monitoring
Methods EPA TO-17
Product Line Carbopack
6

MilliporeSigma™ Supelco™ TSKgel™ G2000HHR-H HT2 HPLC Column, 20 μm Particle Size, Stainless Steel

TSKgel™ Ultra-High Temperature columns are for the analysis of organic-soluble polymers at extremely elevated temperatures.

7

Thermo Scientific™ POROS™ CaptureSelect™ hGH Affinty HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ hGH HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands.

Pore Size 2000 Å
Stationary Phase Growth Hormone affinity ligand, human-specific
Column Type Affinity
Elution Volume Max 29 column volumes
Quantity 1 Column
Material PEEK
For Use With (Application) FPLC
High-throughput Compatibility Automated Protocols
Max. Pressure 2600 psi, 180 bar
Particle Size 20 μm
Matrix POROS
Target Molecule Protein
Product Type Column
Binding Capacity 1.7 mg/mL
Purification Target Protein
Elution Volume Min 14 column volumes
Product Line CaptureSelect, POROS
8

Thermo Scientific™ POROS™ CaptureSelect™ IgA Affinity HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ IgA HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands.

Pore Size 2000 Å
Stationary Phase IgA affinity ligand, human-specific
Column Type Affinity
Elution Volume Max 29 column volumes
Quantity 1 Column
Material PEEK
For Use With (Application) FPLC
High-throughput Compatibility Automated Protocols
Max. Pressure 2600 psi, 180 bar
Particle Size 20 μm
Matrix POROS
Target Molecule Protein
Product Type Column
Binding Capacity 7.6 mg/mL
Purification Target Protein
Elution Volume Min 14 column volumes
Product Line CaptureSelect, POROS
9

Thermo Scientific™ OLIGO™ R3 Reversed Phase Columns

Thermo Scientific™ Oligo™ R3 bulk resin is a rigid polymeric bead designed for reversed-phase chromatography of polynucleotides and other biomolecules.

Shipping Condition Room Temperature
Pore Size 350 Å
Particle Size 30 μm
Stationary Phase Phenyl
Length (Metric) 100 mm
Product Type Analytical Column
Column Type Reversed Phase
Diameter (Metric) 4.6 mm
Volume (Metric) 1.7 mL
Quantity 1 Column
Max. Pressure 2400 psi (170 bar)
Product Line POROS
Column Format Prepacked Column
10

Thermo Scientific™ MAbPac™ SCX-10 RS Monoclonal Antibody Analysis Column, 5μm
GREENER_CHOICE

For high-resolution, high efficiency analysis of monoclonal antibodies (mAbs) and associated variants, use Thermo Scientific™ MAbPac™ SCX-10 RS Monoclonal Antibody Analysis Column, 5μm.

Pore Size Nonporous
Stationary Phase Sulfonate
Phase Ion Exchange
Column Type Ion Exchange
Quantity Each
Green Features FSC
Max. Pressure 7000 psi (480 bar)
Column Format Analytical Column
Particle Size 5 μm
Packing Material Spherical, Nonporous Polymer
Diameter (Metric) 2.1 mm
pH 2 to 12
Technique Bio-LC
Product Line MAbPac
11

MilliporeSigma™ Supelco™ Ascentis™ Express HILIC HPLC Column, 5 μm Particle Size

The bare silica stationary phase of Ascentis™ Express 5 μm HILIC provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase Silica
Phase Reversed Phase
Matrix Silica Phase/Fused-Core Particle Platform
Endcapped No
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
12

MilliporeSigma™ Supelco™ TSKgel™ G2500HXL HPLC Column, 5 μm Particle Size

TSKgel G2500HXL Columns are packed with rigid, porous, polymer beads. They are applied to the non-aqueous separation of oligomers and polymers of less than 20,000 Da.

13

TSKgel™ SuperQ-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel SuperQ-5PW Columns are packed with porous hydrophilic polymer beads which are modified with a strong anion exchanger. Columns are used for the separation and analysis of proteins, oligonucleotides, and other biologically active molecules.

Particle Size 10 μm
Solvents ≤ 20% Organic Solvent
Stationary Phase Ammonium Ion (Quaternary Phase)
Temperature ≤45°C
Length (Metric) 7.5 cm
Ion Exchange Group Cl-
pH 2 to 12
Technique Anion Exchange
Max. Pressure 200 bar
Product Line TSKgel
USP Type L23
14

TSKgel™ Alpha-4000 HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Packed with rigid, porous polymer beads which show minimal shrinkage or swelling in different solvent systems. Columns are best used for the separation of polymers that are soluble in polar organic solvents.

15

TSKgel™ G2000HHR-H HT HPLC Column, 20 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel HT (High Temperature) Columns are for the analysis of organic-soluble polymers at temperatures up to 140°C. They are shipped in o-dichlorobenzene (ODCB), which can be exchanged for 1-chloronaphthalene and trichlorobenzene.