Real Time PCR Reagents and Kits
Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystemsâ„¢ MycoSEQâ„¢ Plus Mycoplasma Detection Kits
The MycoSEQâ„¢ Plus Mycoplasma Detection Kit is a TaqManâ„¢-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.
Invitrogenâ„¢ ROX Reference Dye
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
Invitrogenâ„¢ SuperScriptâ„¢ III Platinumâ„¢ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Invitrogenâ„¢ EXPRESS One-Step Superscriptâ„¢ qRT-PCR Kit, universal
Kit incorporates proven Invitrogenâ„¢ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.
Applied Biosystemsâ„¢ TaqManâ„¢ Vaginal Microbiota Amplification Control
The TaqManâ„¢ Vaginal Microbiota Amplification Control is designed to evaluate the amplification efficacy of the TaqMan Vaginal Microbiota assays. This plasmid serves as a positive control for the TaqMan Vaginal Microbiota Assay collection.
Applied Biosystemsâ„¢ TaqManâ„¢ Fast Reagent Starter Kit
Designed to detect and quantify Eukaryotic 18S rRNA sequence in cDNA samples using the TaqMan 5' nuclease assay, in fast thermal cycling mode.
Applied Biosystemsâ„¢ Precision Plate Holder for Plates, for 7500 Fast System
Precision plate holder for 7500 fast system
Applied Biosystemsâ„¢ TaqManâ„¢ MicroRNA Reverse Transcription Kit
Provides the necessary components for 200 reactions of optimal performance in TaqManâ„¢ MicroRNA Assays.
Applied Biosystemsâ„¢ TaqManâ„¢ Bacillus anthracis Detection Kit
TaqManâ„¢ Bacillus anthracis Detection Kit
Applied Biosystemsâ„¢ TaqManâ„¢ Sample-to-SNPâ„¢ Kit (20 mL sample prep, 10 mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Applied Biosystemsâ„¢ MeltDoctorâ„¢ HRM Reagent Kit
Provides individually packaged PCR components and the MeltDoctorâ„¢ HRM Dye required for high resolution melting analysis.
Applied Biosystemsâ„¢ VetMAXâ„¢-Plus Multiplex One Step RT-PCR Kit
For use with VetMAXâ„¢ Reagents and VetMAXâ„¢ Controls.
Applied Biosystemsâ„¢ Megaplexâ„¢ RT Primers, Human Pool Set v3.0
Megaplexâ„¢ RT Primers, Human Pool Set v3.0