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Applied Biosystems™ TaqMan™ PreAmp Master Mix Kit
Description
- Amplify cDNA targets equally without introducing bias
- Analyze mRNA from any precious sample such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE)
- Stretch as little as 1ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression Assays
- Analyze up to 100 gene expression targets with minimal hands-on time
Order Info
Guaranteed minimum shelf life is 60 days.
Specifications
Specifications
| Content And Storage | Contains 2X TaqMan PreAmp Master Mix (1 mL), TaqMan™ Gene Expression Master Mix (5 mL), and TaqMan™ PreAmp Master Mix Kit Quick Reference Card. Store master mixes at 2-8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
| Detection Method | Primer-probe |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Reaction Speed | Fast or Standard |
| For Use With (Equipment) | 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System |
| Passive Reference Dye | No ROX |
| Product Line | TaqMan |
| Product Type | PreAmp Master Mix Kit |
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Frequently Asked Questions (FAQs)
There is a preamplification protocol included with our TaqMan Sample-to-SNP Kit. This is designed to work with a lysate sample.
The TaqMan PreAmp Master Mix Kit (P/N 4384267) is intended for use with very small quantities of cDNA (1 to 250 ng). This kit allows you to increase the quantity of specific cDNA targets for gene expression analysis using TaqMan Gene Expression Assays. Starting material is increased prior to PCR and the resulting preamplification product is then used for PCR. The TaqMan PreAmp Master Mix Kit enables multiplex preamplification of up to 100 targets at a time and provides unbiased amplification of specific amplicons for analysis with TaqMan Gene Expression Assays. For more detailed information on this kit, please refer to the TaqMan PreAmp Master Mix Kit Protocol (P/N 4384557).
The TaqMan PreAmp Master Mix Kit (P/N 4384267) preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your limited sample into many more real-time PCR reactions. TaqMan PreAmp Master Mix Kit consists of TaqMan PreAmp Master Mix and the TaqMan Gene Expression Master Mix. The two work hand in hand to provide the best results in preamplification and quantitation of mRNA.
For more detailed information on this kit, please refer to the TaqMan PreAmp Master Mix Kit Protocol (P/N 4384557).
TaqMan PreAmp Master Mix is designed for targeted preamplification of up to 100 targets from small amounts of cDNA, while SuperScript IV Single Cell/Low Input cDNA PreAmp Kit allows global preamplification of full-length cDNA from single cells or low input RNA. It provides reagents for cell lysis, reverse transcription and preamplification steps.
For Research Use Only. Not for use in diagnostic procedures.