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Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit

Catalog No. 4366596
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200 Reactions
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4366596 200 Reactions
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Catalog No. 4366596 Supplier Applied Biosystems™ Supplier No. 4366596
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Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.

A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.

Components of this kit are used with the RT primer provided with the TaqMan™ MicroRNA Assay to convert miRNA to cDNA.

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Shipping Conditions: Dry Ice

Specifications

Concentration 10X
Content And Storage Contains 10X RT buffer, dNTP mix w/dTTp (100M total), RNase inhibitor (20U/μL), and MultiScribe™ RT enzyme (50U/μL) for 200 reactions. Store at -15 to -25°C.
Detection Method Primer-probe
GC-Rich PCR Performance High
PCR Method 2-step RT-qPCR
Reaction Speed Fast
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Optimal Reaction Temperature 42°C
Product Line TaqMan
Product Type MicroRNA Reverse Transcription Kit
Quantity 200 reactions
Reverse Transcriptase MultiScribe™
Shipping Condition Dry Ice
Sufficient For 200 Reactions
For Use With (Application) miRNA analysis
Fidelity (vs. Taq) 10 X
Final Product Type cDNA
No. of Reactions 200 Reactions
Reaction Format Separate Components
Sample Type RNA
Size (Final Product) 200 bp or less
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For Research Use Only. Not for use in diagnostic procedures.