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Applied Biosystems™ MeltDoctor™ HRM Reagent Kit DFS Item

Provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis.

Supplier:  Applied Biosystems™ 4425557

Catalog No. 4425557


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Includes: AmpliTaq Gold™ 360 DNA Polymerase, AmpliTaq Gold 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, MeltDoctor HRM Dye (20X)

Description

Description

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.

This kit includes:

  • AmpliTaq Gold™ 360 DNA Polymerase which, when combined with AmpliTaq Gold™ 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets
  • GeneAmp™ dNTP Blend
  • MeltDoctorHRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

Gene Expression Analysis & Genotyping, Genetic Variation Analysis Using Real-Time PCR, Genotyping & Genomic Profiling, High Resolution Melting (HRM) Analysis, Methylation Analysis, Methylation-Sensitive HRM Analysis, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Real Time PCR (qPCR)

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Specifications

This kit includes: AmpliTaq Gold™ 360 DNA Polymerase (2 x 50 μL, each at 5 U/μL), AmpliTaq Gold™ 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, and MeltDoctor™ HRM Dye (20x). The kit provides sufficient materials for 250 20-μL reactions when using 2 U AmpliTaq Gold 360 DNA Polymerase per reaction.

Store at -15° to -25°

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
High
AmpliTaq Gold 360 DNA Polymerase
7500 Fast System, 7900HT Fast System
High Resolution Melting (HRM), SYTO 9
Hrm Reagent Kit
Real Time PCR (qPCR)
HRM
qPCR
HRM (High Resolution Melting) Analysis, Mutation Scanning
SNPs (Unknown or Numerous), Epigenetic Targets
MeltDoctor
1 kit
DNA (Genomic), cDNA
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For Research Use Only. Not for use in diagnostic procedures.