Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions

Primer is suitable for use in first-strand cDNA synthesis with reverse transcriptase

Serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase

Truly random primers suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates

Optimze cDNA synthesis with these random hexamer primers, oligo(dT)₁₈ primers and anchored oligo dT primers.

Primers for PCR amplification that complement many vectors

Use for first-strand cDNA synthesis with a reverse transcriptase.

Template primers

Sequence Detection Primer

Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C

Used for priming and reverse transcription of polyadenylated (poly A+) mRNA

Provided at a stock concentration of 50μM

Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions

Thermo Scientific™ Oligo(dT)₁₈ Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.

Ambion Lambda DNA is digested to completion with Hind III

Ambion pUC 19 DNA is digested to completion with Sau3A I

Accurately sequence DNA with M13 pUC sequencing primers, single-stranded oligonucleotides with 5'-hydroxyl and 3'-hydroxyl ends.

Sequence DNA fragments inserted into the MCS of various pUC19 based cloning vectors with M13 pUC sequencing primers, single-stranded oligonucleotides.

Sequence DNA fragments inserted into the MCS of various pUC19-based cloning vectors with M13 pUC sequencing primers, single-stranded oligonucleotides.

When incorporated into DNA, 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups

Ideal for use as substrates as part of in vitro transcription reactions

Provided at a stock concentration of 50μM, and functionally tested using the RETROscript™ Kit

Ideal for use as substrates as part of in vitro transcription reactions

Contain mixtures of linearized plasmids ready for use as templates as part of in vitro transcription reactions for synthesis of labeled RNA molecular size standards

Ideal for use as substrates as part of in vitro transcription reactions

Ambion Modified nucleotides confer unique characteristics to the RNA molecules into which they are incorporated

Primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (either dG, dA, or dC) and then by dN (dA, dT, dG, or dC)

Contain mixtures of linearized plasmids ready for use as templates during in vitro transcription reactions for synthesis of labeled RNA molecular size standards

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.