Thermo Scientific™ pJET1.2 Sequencing Primers
Primers for sequencing of DNA fragments inserted into Eco32I site within the eco47IR gene of pJET1.2 and for colony screening by PCR.
Manufacturer: Thermo Scientific™ SO511
Thermo Scientific sequencing primers are single-stranded oligonucleotides with 5'-hydroxyl and 3'-hydroxyl ends. pJET1.2 sequencing primers flank the Eco32I site in the eco47IR gene of positive selection cloning vector pJET1.2. All primers are supplied as 10 µM aqueous solutions.
- Sequencing of DNA fragments inserted into Eco32I site within the eco47IR gene of pJET1.2
- Colony screening by PCR
|pJET™1.2 forward sequencing primer, 24-mer|
|0.2 A260, (6.7µg)|