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Thermo Scientific™ pJET1.2 Sequencing Primers


Primers for sequencing of DNA fragments inserted into Eco32I site within the eco47IR gene of pJET1.2 and for colony screening by PCR.

Manufacturer: thermo scientific™  SO511

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Catalog No. FERSO511

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Description & Specifications

Specifications

Product Type pJET™1.2 forward sequencing primer, 24-mer
Quantity 0.2 A260, (6.7µg)

Thermo Scientific sequencing primers are single-stranded oligonucleotides with 5'-hydroxyl and 3'-hydroxyl ends. pJET1.2 sequencing primers flank the Eco32I site in the eco47IR gene of positive selection cloning vector pJET1.2. All primers are supplied as 10 µM aqueous solutions.

Applications

  • Sequencing of DNA fragments inserted into Eco32I site within the eco47IR gene of pJET1.2
  • Colony screening by PCR