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Invitrogen™ Lambda Hind III dsDNA Markers

Catalog No. AM7720
Encompass
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200 ug
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Catalog No. Quantity
AM7720 200 ug
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Catalog No. AM7720 Supplier Invitrogen™ Supplier No. AM7720
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Generates fragments of the sizes 23,130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp.

Generates fragments of the sizes 23,130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp. These DNA molecular mass markers are supplied with a protocol describing easy labeling of the markers with radioisotopes. They can also be stained with ethidium bromide. One tube contains 200 μg.

Order Info

Shipping Conditions: Room Temperature

Specifications

Product Type DNA Marker
Content And Storage Store at –20°C.
Gel Compatibility Agarose Gels
Shipping Condition Room Temperature
Size Range 125 to 23.1 kb
Concentration 0.5 mg/mL
Quantity 200 ug
Product Line Ambion
Form Liquid
How can I quantitate DNA on an agarose gel?

For fragments between 100 bp and 2,000 bp, use the Low DNA Mass Ladder. 4 µL of the Low DNA Mass Ladder corresponds to 10, 20, 40, 80, 120, and 200 ng respectively of fragments, 100, 200, 400, 800, 1,200 and 2,000 bp.

For fragments between 1,000 bp and 10,000 bp use the High DNA Mass Ladder. 4 µL of the High DNA Mass Ladder corresponds to 20, 40, 60, 80, 120, 200 ng respectively of fragments 1,000, 2,000, 3,000, 4,000, 6,000, and 10,000 bp.

To calculate the amount of DNA in 1 µg of the Lambda/Hind III standard:
(size of band in bp/48503 bp) X 1 µg = µg of DNA in band A

For example, to calculate the amount of DNA in the 564 bp band of the Lambda/Hind III standard:
(564/48,503) X 1 µg = 0.011 µg or 11 ng of DNA in the 564 bp band.

For Research Use Only. Not for use in diagnostic procedures.