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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (240)
Real Time PCR Assays and Arrays (231)
Real Time PCR Reagents and Kits (184)

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1

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0  DFS Item

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan
2
Promotions Available

Applied Biosystems™ TaqMan™ Gene Expression Master Mix  DFS Item

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

Polymerase AmpliTaq Gold DNA Polymerase
Purity or Quality Grade UP (Ultra Pure)
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
3

Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay  DFS Item

The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results.

Shipping Condition Dry Ice
Format Kit
Label or Dye VIC
Species Not Species-Specific
Product Line VetMAX, Xeno
4

Applied Biosystems™ SYBR™ Select Master Mix  DFS Item

Cost-effective solution for real-time PCR applications.

GC-Rich PCR Performance High
Content And Storage The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq DNA Polymerase
Format Vial
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Label or Dye SYBR GreenER
Hot Start Built-In Hot Start
Sufficient For 100 Reactions of 20 μL
Product Line SYBR
5

Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit, 100 reactions  DFS Item

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.

6
Promotions Available

Applied Biosystems™ SYBR™ Green Universal Master Mix  DFS Item

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Standard
Label or Dye SYBR Green
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line SYBR
Detection Method SYBR
7
Promotions Available

Applied Biosystems™ TaqMan™ Gene Expression Assays  DFS Item
GREENER_CHOICE

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR or digital PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Content And Storage 1 tube containing 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15°C to -25°C.
Fidelity (vs. Taq) 60 X
Product Type Gene Expression Assay
Reaction Speed Fast
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
8
Promotions Available

Applied Biosystems™ TaqMan™ Universal PCR Master Mix  DFS Item

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Product Type Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
9
Promotions Available

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR  DFS Item

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Shipping Condition Wet Ice
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Dual-Lock Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System, Applied Biosystems AmpQuest 96
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) Medium
Target Specificity cDNA or gDNA only
Product Line PowerUP, SYBR
10
Promotions Available

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR  DFS Item

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Shipping Condition Dry Ice
Content And Storage • PowerTrack SYBR Green Master Mix
• 40X Yellow Sample Buffer

Store at -20±5°C in the dark.
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 8 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity cDNA or gDNA only
Product Line Applied Biosystems
11

Applied Biosystems™ QuantStudio™ Absolute Q™ Isolation Buffer

The QuantStudio Absolute Q Isolation Buffer is intended to supplement the materials supplied in the QuantStudio Absolute Q MAP16 Plate Kit.

12

Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)

Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.

13

Applied Biosystems™ MeltDoctor™ HRM Master Mix  DFS Item

Contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

GC-Rich PCR Performance High
Form Liquid
For Use With (Application) Genotyping
PCR Method qPCR
Detection Method HRM
Polymerase AmpliTaq Gold 360 DNA Polymerase
Genotyping Target SNPs (Unknown or Numerous), Epigenetic Targets
Product Type HRM Master Mix
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Label or Dye High Resolution Melting (HRM),SYTO 9
Technique HRM (High Resolution Melting) Analysis, Mutation Scanning
Product Line MeltDoctor
14

Thermo Scientific™ Maxima Probe qPCR Master Mixes

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

15

Applied Biosystems™ Path-ID™ qPCR Master Mix with Manual  DFS Item

Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.

Content And Storage 2X qPCR Master Mix is stored at –20°C. Nuclease-free water may be stored at any temperature.
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
Concentration 2X
For Use With (Application) Animal Health,Pathogen Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Tube
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Fast or Standard
Hot Start Built-In Hot Start
Product Line Ambion, Path-ID