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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Product Line HisPur
2

Thermo Scientific™ Pierce™ High-Capacity EDTA-Compatible Ni-IMAC Resin

Pierce High-Capacity EDTA-Compatible Ni-IMAC Resin is designed for the purification of over-expressed His-tagged proteins from cell culture media.

Binding Property Chelator Stable
3

Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 3 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

4

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Pierce Anti-DYKDDDDK Spin Columns provide a fast, convenient method for purification of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells.

Shipping Condition Wet Ice
Stationary Phase Anti-DYKDDDDK monoclonal antibodies
Product Type Affinity Resin
5

Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide

Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports

Shipping Condition Wet Ice
Matrix Affinity Resin
Format Lyophilized Powder
Form Lyophilized Powder
Protein Form Recombinant
Product Type Peptides
For Use With (Application) Protein Purification and Isolation
Product Line Pierce
6

Thermo Scientific™ HisPur™ Cobalt Spin Columns, 3 mL

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

7

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

8

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (Multi-species) Conjugate
GREENER_CHOICE

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (ms) Conjugate consists of a 13 kDa recombinant single domain antibody fragment that specifically binds to the Fc part of IgG from multiple species.

Ligand Type Antibody
Type Conjugate
Target IgG (Fc region)
Content And Storage Store at -5°C to -30°C.
Form Liquid
Label or Dye Biotin & Other Haptens
Product Line CaptureSelect
9

MilliporeSigma™ Novagen™ Ekapture™ Agarose

Intended for rapid affinity-based capture.

Type Agarose
Content And Storage 2°C to 8°C
10

Cytiva HiScreen™ Capto™ S, Capto™ Q and Capto™ DEAE Prepacked IEX Columns

Designed for method optimization and parameter screening

Buffer 1M Acetic Acid, 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Column Type Ion Exchange Column
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Modern large-scale manufacturers for fast, efficient and cost-effective capture and intermediate Protein purification, optimization and parameter screening
Max. Pressure 3 bar
Particle Size 45 to 165μm
Flow Rate <700 cm/hr.
Support Type Highly cross-linked Agarose with Dextran surface extender
Height (English) 3.93 in.
11

Thermo Scientific™ POROS™ Benzyl Hydrophobic Interaction Chromatography (HIC) Resin
GREENER_CHOICE

Thermo Scientific™ POROS™ Benzyl Hydrophobic Interaction Chromatography (HIC) Resin is a bioprocess high-performance resin engineered for improved impurity removal during large-scale downstream purification of biomolecules.

Shipping Condition Ambient
Particle Size 50 μm
Ligand Type Benzyl/Aromatic
Stationary Phase Low-Density Benzyl/Aromatic
Content And Storage Store at 2°C to 30°C. Do not freeze.
Type Resin
Product Line POROS
12

Thermo Scientific™ POROS™ AL 20 μm Prepacked Immobilized Aldehyde Affinity Columns
GREENER_CHOICE

Thermo Scientific™ POROS™ Prepacked Immobilized Aldehyde Affinity Column. POROS AL is based on an aldehyde functional group designed for the immobilization of proteins through free amine groups.

Particle Size 20 μm
Shipping Condition Room Temperature
Pore Size 2000 Å
Stationary Phase Aldehyde
Type Pre-packed Column
Content And Storage Store at room temperature.
Format Column
Column Type Activated
Quantity 1 Column
Material PEEK
Product Line POROS
13

MilliporeSigma™ D-Tube™ Dialyzer Maxi, MWCO 6-8 kDa

Used for dialysis and electroelution of proteins, RNA, DNA, and oligonucleotides from polyacrylamide or agarose gels

14
Promotions Available

Sartorius Sartoclear Dynamics™ Lab V Filtration Kit

Sartorius Sartoclear Dynamics™ Lab V Kits designed for the clarification and sterile filtration of mammalian cell culture with diatomaceous earth as filter aid.

Product Type Vacuum Filtration Kit
15

Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Matrix
GREENER_CHOICE

Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix.

Particle Size 65 ±10 μm
Ligand Type C-tagXL affinity ligand
Flow Rate 50 to 200 cm/hr. (Up to 2 bar)
Stationary Phase Anti-EPEA tag
Type Affinity Matrix
Matrix Agarose-based, epoxide-activated
Form Liquid Suspension
Binding Capacity 400 nmol/mL
Product Line CaptureSelect