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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1

Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel

Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration

2

Cytiva Sephadex G-50 Medium, 500g

Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.

3

Cytiva Sephadex™ G50 Fine Media

Suitable for gel filtration

Height (Metric) 10cm
Content And Storage 4 to 30°C
Bed Volume 9 to 11mg/g
pH Range 2 to 10
Diameter (Metric) Inner 5cm
For Use With (Application) For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyesFor removeing small nucleotides from longer chain nucleic acids
Max. Pressure min 150 cm/h, bed height 10 cm, column 5 cm i.d.
Particle Size 34 to 208 μm in wet form, 20 to 80 μm in dry form
Flow Rate < Darcy´s Law
Format Solid Bead
Product Type Sephadex G50 Media
Sample Type DNA
Support Type Cross-linked Dextran
Height (English) 3.93 in.
4

Cytiva Sephadex™ G50 Medium Media

Suitable for gel filtration

Particle Size 100 to 300 μm in wet form, 50 to 150 μm in dry form
Height (Metric) 10cm
Bed Volume 9 to 11mg/g
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G50 Media
Sample Type DNA
Diameter (Metric) Inner 5cm
Support Type Cross-linked Dextran
Height (English) 3.93 in.
5

Cytiva Sephadex™ G-50 and G-100 DNA Grade Agarose Gels

Used for purification of DNA from small molecules. Cytiva illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration.

Content And Storage 4° to 30°C
Format Beaded Gel
pH Range 2 to 10
Product Type G-50 and G-100 DNA Grade Agarose Gels
For Use With (Equipment) Auto Seq G-50 column, ProbeQuant G-50 micro column, MicroSpin G-50 column and NICK column
Sample Type DNA
Support Type Cross-linked Dextran with Epichlorohydrin
6

Cytiva Sephadex™ G50 Superfine Media

Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.