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Gene Reporter Systems

Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.
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1

MilliporeSigma™ Calbiochem™ Coelenterazine

A cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescence probe of the superoxide anion.

2

Invitrogen™ Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System  DFS Item

Thermo Scientific Luc-Screen Extended-Glow Luciferase Reporter Gene Assay System with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the

Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Luciferase
Substrate Type Luciferase Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Luciferin
Assay Luciferase Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type Luciferase Reporter Gene Assay System
Sample Type Cell Cultures
Product Line Luc-Screen, NovaBright
4

Invitrogen™ RadPrime DNA Labeling System  DFS Item

Ideal for rapid preparation (under 10 min) of high-specific activity 32 P-labeled probes for the detection of DNA and RNA

5

Invitrogen™ BLOCK-iT™ U6 RNAi Entry Vector Kit  DFS Item

Provides a simple, streamlined approach to cloning short hairpin RNA (shRNA) sequences for testing in transient transfections for RNA interference (RNAi)

6

Invitrogen™ BioPrime™ Array CGH Genomic Labeling System  DFS Item

A high-performance labeling kit that enable reproducible labeling of genomic DNA samples for array-based Comparative Genomic Hybridization (CGH)

7

Invitrogen™ Human Cot-1 DNA™-Fluorometric QC  DFS Item

Quantifying by fluorometry provides greater data accuracy

8

Invitrogen™ Mouse Cot-1 DNA  DFS Item

Mouse DNA predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the B1, B2, and L1 family members

9

Invitrogen™ Biotin-14-dCTP  DFS Item

Used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling

10

Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells  DFS Item

Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Beta-Galactosidase
Substrate Type Beta-Gal Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Galacton-Star™
Assay β-Gal (lacZ) Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type β-Galactosidase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
Product Line Gal-Screen, NovaBright
12

Invitrogen™ MessageAmp™ II aRNA Amplification Kit  DFS Item

Amplifies as little as 100pg of input RNA for GeneChip™ analysis

Format Kit
Reverse Transcriptase ArrayScript™
Includes Label or Dye No
Product Type aRNA Amplification Kit
Sample Type Poly(A+) RNA, Total RNA
Product Line Ambion, MessageAmp
13

Invitrogen™ Human Cot-1 DNA™  DFS Item

Effective library-screening probe for somatic-cell hybrid libraries and flow-sorted chromosome libraries made from somatic-cell hybrids

14

Invitrogen™ λ DNA (cIind 1 ts857 Sam 7)  DFS Item

λ DNA (cIind 1 ts857 Sam 7) is isolated from a dam+ dcm+ E coli LE597 lysogen

15

Invitrogen™ NovaBright™ Phospha-Light™ EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection  DFS Item

Chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase or the non-secreted placental isozyme PLAP.

Substrate CSPD™
Substrate Properties Chemical Substrate
Content And Storage Component solution containing CSPD™ substrate and chemiluminescence enhancer (Store at 2°C to 6°C), placental alkaline phosphatase positive control (Store at ≤ -20°C)
Format 96-well Plate
For Use With (Equipment) Luminometer (Microplate)
Product Type Phosphatase Assay
Target Enzyme Placental Alkaline Phosphatase
Substrate Type AP (Alkaline Phosphatase) Substrate
High-throughput Compatibility High-throughput Compatible
Product Line NovaBright
Detection Method Chemiluminescence