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Chinese Hamster Ovary Cell Culture Media

Liquid solutions, reagents, and dry compounds designed to support the growth, proliferation, and transfection of Chinese hamster ovary (CHO) cells; various formulations and volumes available.
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1

Gibco™ ExpiCHO™ Expression Medium
GREENER_CHOICE

A GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells.

Shipping Condition Room Temperature
Cell Line ExpiCHO-S™, CHO-S, SFM Adapted
Content And Storage Storage conditions: in the dark at 2-8°C
Shipping conditions: ambient
Shelf life: 12 months from date of manufacture
With Additives GlutaMAX
Product Type CHO Expression Medium
Form Liquid
Without Additives No Phenol Red
Classification Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type Suspension Cell Culture
Product Line ExpiCHO
Serum Level Serum-free
2

Gibco™ High-Intensity Perfusion CHO Medium

Gibco High-Intensity Perfusion (HIP) CHO Medium is an innovative, chemically defined, animal origin-free (AOF), cGMP medium.

Cell Line CHO-S, CHO-GS, CHO-K1, CHO-DG44
Content And Storage Dry format medium. Store in the dark and dry at 2–8°C.
With Additives Glucose
Product Type Perfusion CHO Medium
Form Dry Powder
Without Additives No Glutamine
Manufacturing Quality cGMP
Culture Type Suspension Cell Culture
Shelf Life 18 months
Concentration 34.92 g/L
Sterility Non-sterile
3

Gibco™ Efficient-Pro™ Medium

Gibco™ Efficient-Pro™ Media delivers superior product performance, improved product titer, better protein quality and lot-to-lot consistency for more control and predictability. Chemically defined and completely scalable, available in liquid or AGT™ formats for sustained cell viability.

Shipping Condition Room temperature
Cell Line CHO-DG44, CHO-S, CHO-K1, GS-CHO
Content And Storage Store in the dark at 2–8°C.
Form Liquid
Classification Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type Mammalian Cell Culture
Green Features Sustainable packaging
4

Gibco™ Efficient-Pro™ AGT™ Medium

Gibco™ Efficient-Pro™ Media delivers superior product performance, improved product titer, better protein quality and lot-to-lot consistency for more control and predictability. Chemically defined and completely scalable, available in Gibco™ AGT™ formats for sustained cell viability.

Shipping Condition Room temperature
Cell Line CHO-DG44, CHO-S, CHO-K1, GS-CHO
Content And Storage Store in the dark at 2–8°C.
Form Powder (AGT)
Classification Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type Mammalian Cell Culture
Green Features Sustainable packaging
6

Cytiva HyClone™ PF-CHO™ LS and PF-CHO MPS Media

Support growth of multiple CHO cell clones and production of a variety of recombinant proteins. HyClone™ PF CHO™ LS and PF CHO MPS Media is a protein-free media developed through Metabolic Pathway Design.

Certifications/Compliance cGMP
8

Cytiva HyClone™ ActiPro Powdered Cell Culture Media

Formulated to provide high yields of recombinant proteins in bioprocesses using Chinese hamster ovary (CHO) cell lines.

Content And Storage 2 to 8°C, away from light and moisture
With Additives Poloxamer 188
Form Powder
Without Additives No Glutamine
Shelf Life 18 months
Sterility Non-sterile
9

Gibco™ CHO-S-SFM II, with hypoxanthine and thymidine
GREENER_CHOICE

CHO-S-SFM II, with hypoxanthine and thymidine

Shipping Condition Room Temperature
Cell Line CHO
Content And Storage Store in refrigerator (2–8°C) and protect from light.
Format Bottle(s)
Included Antibiotics No antibiotics
Product Type Serum Free Medium (SFM)
Form Liquid
Endotoxin Level Low
Classification Low Protein,Serum-free
Culture Type Suspension Cell Culture
Concentration 1 X
Product Line Gibco
Serum Level Serum-free
10

Gibco™ CD OptiCHO™ AGT™ Medium

CD OptiCHO™ AGT™ Medium

Shipping Condition Wet Ice
Cell Line CHO
Culture Environment CO2
Purity or Quality Grade Certified
Inorganic Salts Magnesium, Sodium Phosphate
Form Powder (AGT)
Without Additives No Glutamine,No Phenol Red
Endotoxin Level Low
Sterility Non-sterile
Included Antibiotics No antibiotics
Remarks Medium does not support wild type DG44 cells
With Additives High Glucose,Sodium Pyruvate,Sodium Bicarbonate
Product Type CD OptiCHO AGT Medium
Classification Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type Suspension Cell Culture
Product Line AGT, Gibco
Serum Level Serum-free
11

Gibco™ CD CHO Medium  DFS Item

Shipping Condition Room Temperature
Cell Line CHO
Content And Storage Store in refrigerator (2–8°C) and protect from light.
Form Liquid
Without Additives No Glutamine,No Phenol Red,No Thymidine,No Hypoxanthine
Endotoxin Level Low
Concentration 1 X
Format Bottle
Included Antibiotics No antibiotics
With Additives Sodium Pyruvate,Sodium Bicarbonate
Product Type CD CHO Medium
Classification Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type Suspension Cell Culture
Species Hamster
Product Line Gibco
Serum Level Serum-free
12

CD CHO Medium

Shipping Condition Room Temperature
Cell Line CHO
Content And Storage Store at 2-8°C in the dark.
Culture Environment CO2
Purity or Quality Grade Cell Culture Grade
Form Liquid
Without Additives No Glutamine,No Phenol Red,No Thymidine,No Hypoxanthine
Endotoxin Level Low
Concentration Ratio 1 :1
Concentration 1 X
Sterility Sterile-filtered
Cell Type Ovarian Cells
Format Universal Bag
Included Antibiotics No antibiotics
With Additives Sodium Pyruvate,Sodium Bicarbonate,Non Essential Amino Acids (NEAA)
Product Type CD CHO Medium
Classification Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type Suspension Cell Culture
Product Line Gibco
Serum Level Serum-free
13

Cytiva™ HyClone™ ActiSM™ Cell Culture Media

A chemically defined ADCF cell culture medium optimized for high-yield protein production in CHO cell culture processes.

14

Cytiva HyClone™ HyCell™ CHO Medium, Liquid

Metabolically designed for high cell yield and recombinant protein production

15

Cytiva HyClone™ ActiCHO™ P Powdered Media

Hydrolysate-free and animal-derived component-free (ADCF)

Content And Storage 2 to 8°C, away from light and moisture
With Additives Poloxamer 188
Form Powder
Without Additives No Glutamine,No Insulin
Shelf Life 36 months
Sterility Non-sterile