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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

MilliporeSigma™ Calbiochem™ Trypsin Porcine MS Grade

A chemically modified trypsin that is resistant to autolysis and degradation

3

MilliporeSigma™ ESGRO Complete™ Accutase™ Cell Detachment Solution

a cell detachment solution of proteolytic and collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.

4

Gibco™ Cell Dissociation Buffer, enzyme-free, PBS  DFS Item

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS).

5

Gibco™ CTS™ Versene™ Solution  DFS Item

CTS Versene Solution is a cGMP-manufactured EDTA solution for use as a gentle non-enzymatic cell dissociation reagent.

6

Gibco™ TrypLE™ Select Enzyme (10X), no phenol red  DFS Item
GREENER_CHOICE

TrypLE™ Select Enzyme (10X), no phenol red

Chelators EDTA
Purity or Quality Grade Cell Culture Grade
Inorganic Salts No Calcium, No Magnesium, No Sodium Phosphate
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 10 X
Osmolality 270 - 320 mOsm/kg
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
pH 6.9 to 7.4
Shelf Life 24 Months
Product Line Gibco
7

Corning™ Accutase™ Cell Detachment Solution, Liquid

Efficient, with gentle detachment of adherent cells within minutes

Content And Storage Store at -20°C.
Form Liquid
Formulation 1 X Accutase enzymes prepared in Dulbeccos PBS (0.2g/L KCl, 0.2g/L KH2PO5, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5 mm EDTA·4Na and 3 mg/L Phenol Red.
Sterility Sterile
8

Invitrogen™ Accutase - Enzyme Cell Detachment Medium

Invitrogen™ Accutase Enzyme Cell Detachment Medium performs exceptionally well in detaching cells for analysis of cell surface markers and virus growth assay.

9

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells.

10

MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x)

Used to dissociate primary cells, tissues, and tumors with an increased plating efficiency. MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x) is PBS based formulation of chelating agents that contain agents to stabilize their activity on cells.

Content And Storage Store at room temperature or at 4°C.
Product Type Cell Culture Dissociation Reagent
Form Liquid
Formulation Chelating and stabilizing agents
Shelf Life 2 Years
Concentration 1 X
For Use With (Application) Cell Dissociation
Sterility Sterile
11

MilliporeSigma™ Enzyme Free Cell Dissociation Solution Hank's Based (1x)

Hank's based formulation of chelating agents and agents to stabilize their activity on cells

Content And Storage 4°C
Formulation Chelating and stabilizing agents
12

Gibco™ Defined Trypsin Inhibitor  DFS Item
GREENER_CHOICE

Animal origin-free

13

Gibco™ Trypsin/EDTA Solution (TE)  DFS Item
GREENER_CHOICE

With EDTA and phenol red

14

MilliporeSigma™ Xeno-Free 0.05% Trypsin/0.02% EDTA Kit

Useful for routine detachment of cells from standard tissue plasticware and adhesion coated plasticware under animal-free conditions..

15

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.