Hot Start PCR Reagents and Kits
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Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | DNA Polymerase |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Conditions d’expédition | Room Temp or Wet Ice |
| À utiliser avec (application) | Hot-start PCR |
| Polymérase | AmpliTaq Gold 360 DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Tube |
| Activité exonucléasique | 5' - 3' |
| Hot Start | Built-In Hot Start |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
Applied Biosystems™ True Allele™ PCR Premix
Applied Biosystems True Allele PCR Premix is a PCR master mix that contains AmpliTaq Gold DNA Polymerase, dNTPs, MgCl2, and buffer.
Invitrogen™ Platinum™ II Green PCR Buffer (5X)
Invitrogen 5X Platinum II Green PCR Buffer for fast and easy PCR
Invitrogen™ AccuPrime™ Taq DNA Polymerase System
The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.
| GC-Rich PCR Performance | Low |
|---|---|
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | DNA Polymerase System |
| En surplomb | 3'-A |
| Méthode PCR | 1-step RT-qPCR,Multiplex qPCR |
| Concentration | 10X |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
| Polymérase | AccuPrime Taq DNA Polymerase |
| Fidélité (par rapport à Taq) | 2X |
| Hot Start | Built-In Hot Start |
| Taille (produit final) | 5 kb or less |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
| GC-Rich PCR Performance | Low |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Activité exonucléasique | 5' - 3' |
| Type de produit | DNA Polymerase With Buffer I |
| Hot Start | Built-In Hot Start |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| À utiliser avec (application) | Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
| Polymérase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Activité exonucléasique | 5' - 3' |
| Type de produit | DNA Polymerase |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Taille (produit final) | 5 kb or less |
Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
| GC-Rich PCR Performance | High |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Format | Tube |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| Taille (produit final) | 5 kb or less |
| À utiliser avec (application) | Hot-start PCR |
Applied Biosystems™ Platinum™ Multiplex PCR Master Mix
The Platinum Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR.
| Capacité multiplex | Up to 20-plex |
|---|---|
| Polymérase | Platinum Taq DNA Polymerase |
| GC-Rich PCR Performance | Low |
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Standard |
| Format de réaction | SuperMix or Master Mix |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| Taille (produit final) | 2.5 kb or less |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR,Multiplex PCR |
Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | Standard |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | PCR Master Mix |
| En surplomb | 3'-A |
| Concentration | 2X |
| Conditions d’expédition | Room Temp or Wet Ice |
| À utiliser avec (application) | Cloning,Hot-start PCR |
| Polymérase | AmpliTaq Gold 360 DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Tube |
| Activité exonucléasique | 5' - 3' |
| Hot Start | Built-In Hot Start |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
| Polymérase | AccuPrime GC-Rich DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidélité (par rapport à Taq) | 2X |
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Taille (produit final) | 5 kb or less |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
Thermo Scientific™ Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
| Polymérase | Phire Hot Start II DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Medium |
| Fidélité (par rapport à Taq) | 2X |
| Vitesse de réaction | Fast |
| Format de réaction | Separate Components |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Matériau de départ | DNA |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Cloning,Hot-start PCR |
Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)
Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.
| GC-Rich PCR Performance | Medium |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Standard |
| Format | Tube |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Hot Start PCR Master Mix |
| Hot Start | Built-In Hot Start |
| En surplomb | 3'-A |
| Concentration | 2X |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
| Polymérase | Platinum II Taq Hot-Start DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Fast or Standard |
| Format de réaction | Separate Components |
| Type de produit | Hot Start DNA Polymerase |
| En surplomb | 3'-A |
| Concentration | 2X |
| Taille (produit final) | 5 kb or less |
Invitrogen™ PCR Master Mix Starter Pack
The PCR Master Mix Starter Pack is composed of three different PCR master mixes for easy evaluation of their suitability for use in your research.
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase
Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
| Polymérase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Hot Start | Built-In Hot Start |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| À utiliser avec (application) | Hot-start PCR |