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Hot Start PCR Reagents and Kits

In hot start PCR, key reagents are prevented from reacting in the PCR mixture until optimal temperatures are met. This reduces non-specific DNA amplification that occurs naturally at lower temperatures, increasing product yield, specificity, and sensitivity.
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1

Thermo Scientific™ Phire Hot Start II DNA Polymerase

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.

Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
2

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
3

Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
4

Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

Shipping Condition Dry Ice
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
For Use With (Application) Hot-start PCR
5

Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)  DFS Item

Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
Starting Material DNA
For Use With (Application) Hot-start PCR
6

Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase  DFS Item

Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

Polymerase Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type Hot Start DNA Polymerase
Reaction Speed Fast or Standard
Overhang 3'-A
Concentration 2X
7

Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)  DFS Item

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Hot Start Built-In Hot Start
Concentration 2X
For Use With (Application) Hot-start PCR
8

Invitrogen™ Platinum™ Taq Green Hot Start DNA Polymerase  DFS Item

Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer.

9

Promega GoTaq™ MDx Hot Start Polymerase  DFS Item

GoTaq™ MDx Hot Start Polymerase contains GoTaq™ MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity.

Activation Time 2 min.
10

Promega GoTaq™ Hot Start Polymerase: 5X  DFS Item

GoTaq™ Hot Start Polymerase contains the high-performance GoTaq™ DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two minutes.

Activation Time 2 min.
Concentration 5 U/μL
11

Promega GoTaq™ G2 Hot Start Polymerase  DFS Item

GoTaq™ G2 Hot Start Polymerase is supplied with 5X Green GoTaq™ Flexi Buffer, 5X Colorless GoTaq™ Flexi Buffer and 25mM MgCl2.

Polymerase GoTaq G2
Content And Storage −30° to −10°C
Sample Type Hot Start Polymerase
Concentration 5 U/μL
12

Promega GoTaq™ G2 Hot Start Colorless Master Mixes  DFS Item

GoTaq™ G2 Hot Start Master Mixes are ready-to-use mixes containing GoTaq™ G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium

Content And Storage −30° to −10°C
Sample Type Colorless master mix
13

Promega GoTaq™ G2 Hot Start Green Master Mixes  DFS Item

GoTaq™ G2 Hot Start Master Mixes are ready-to-use mixes containing GoTaq™ G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium

Content And Storage −30° to −10°C
Sample Type Green master mix
14

Promega GoTaq™ Hot Start Polymerase: Colorless Master Mix, 2X  DFS Item

GoTaq™ Hot Start Master Mixes are all-in-one master mixes (just add template and primers) that contain the high-performance GoTaq™ DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two

Activation Time 2 min.
Concentration 5 U/μL
For Use With (Application) Cloning
15

Promega GoTaq™ Hot Start Polymerase: Green Master Mix, 2X  DFS Item

GoTaq™ Hot Start Master Mixes are all-in-one master mixes (just add template and primers) that contain the high-performance GoTaq™ DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two

Activation Time 2 min.
Concentration 5 U/μL