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Promega™ GoTaq™ Hot Start Polymerase: Green Master Mix, 2X

Combines the robust amplification and sensitivity of GoTaq DNA Polymerase with hot-start technology to deliver even greater specificity

$109.00 - $865.00

Specifications

Activation Time 2 min.
Concentration 5U/μL
For Use With (Application) Hot-start PCR, complex genomic templates, T-vector cloning, two-step RT-PCR
Includes 2X Hot start green master mix (2 x 1250μL), Nuclease free Water (2 x 1250μL)
Storage Requirements -20°C
View More Specs
Includes:  GoTaq Hot Start Polymerase, buffer, dNTPs, and MgCl2, and supplied with nuclease-free water.
Products
Catalog Number Mfr. No. No. of Reactions Price Quantity    

PR-M5123

 
promega™
M5123
1000.0 Each for $865.00

PR-M5122

 
promega™
M5122
100.0 Each for $109.00
Description & Specifications

Specifications

Activation Time 2 min.
Concentration 5U/μL
For Use With (Application) Hot-start PCR, complex genomic templates, T-vector cloning, two-step RT-PCR
Includes 2X Hot start green master mix (2 x 1250μL), Nuclease free Water (2 x 1250μL)
Storage Requirements -20°C

  • Proprietary antibody bound to the polymerase blocks polymerase activity and permits room-temperture setup, but only requires a two-minute activation at 94°-95°C
  • GoTaq Flexi Reaction Buffers contain a compound that increases sample density and serves as both reaction buffer and gel-loading solution, saving time in post-PCR anaylsis
  • Green GoTaq Flexi Buffer contains two dyes that separate during electrophoresis to facilitate direct-to-gel analysis of PCR products
  • Enzyme exhibits 5' to 3' exonuclease activity

Hot-start PCR, complex genomic templates, T-vector cloning, two-step RT-PCR

U.S. Patent No. 6,242,235, Australian Patent No. 761757, Canadian Patent No. 2,335,153.
Licensed under U.S. Patent Nos. 5,338,671 and 5,587,287 and corresponding patents in other countries.