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  7. Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Catalog No. F533S
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F533S 100 Reactions
F533L 500 Reactions
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Description

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Thermo Scientific Phusion U Hot Start PCR Master Mix is a convenient 2X formula that contains Phusion U Hot Start DNA Polymerase, nucleotides, and an optimized reaction buffer including MgCl2.

Phusion U DNA Polymerase is a novel engineered high-fidelity enzyme developed using fusion technology. Designed with a proprietary mutation in the dUTP binding pocket of Phusion polymerase, Phusion U polymerase is able to overcome important limitations of proofreading enzymes to incorporate dUTP and read through uracil present in DNA templates.

Phusion U polymerase features properties of Phusion DNA polymerases and offers the ability to amplify long amplicons up to 20 kb and a high specificity with affibody based hot start.

These features make Phusion U Hot Start DNA Polymerase an excellent choice for such applications as amplification of bisulfite-converted or damaged DNA as well as use of carryover contamination control.

Highlights

  • High-fidelity DNA polymerase (25X Taq)
  • Engineered to incorporate dUTP and amplify uracil containing templates
  • Hot start for reduced nonspecific amplification and primer degradation
  • Only template and primers need to be added for use
  • Short extension times (15–30 s/kb)

Applications

  • Amplification of bisulfite-converted DNA
  • Amplification of damaged or aged DNA
  • Carry-over contamination control
  • Uracil-excision based (USER) cloning methods

Using Phusion DNA Polymerases

  • Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

Concentration 2X
Content And Storage • Phusion U Hot Start DNA Polymerase
• 2X reaction buffer
• 3 mM MgCl2
• 400 μM each of dATP, dTTP, dCTP and dGTP

Master mix is supplied with nuclease-free water.
Store at -20°C.
GC-Rich PCR Performance Medium
Polymerase Phusion U Hot Start DNA Polymerase
Reaction Speed Fast
Product Type Hot Start PCR Master Mix
Quantity 100 reactions
Shipping Condition Dry Ice
For Use With (Application) Hot-start PCR
Fidelity (vs. Taq) 25X
Hot Start Built-In Hot Start
No. of Reactions 100 Reactions
Overhang Blunt
Reaction Format SuperMix or Master Mix
Size (Final Product) 20 kb or less
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For Research Use Only. Not for use in diagnostic procedures.