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Invitrogen™ Platinum™ Taq DNA Polymerase, high fidelity DFS Item

Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.

Supplier:  Invitrogen™ 11304102

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Catalog No. 11304102

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.


Includes: Platinum Taq DNA Polymerase High Fidelity (1000μL, at 5U/μL) is supplied with a bottle (50mL) of 10X High Fidelity Buffer (600mM Tris-SO4 (pH 8.9), 180mM (NH4)2SO4), and a bottle (25mL) of 50mM MgSO4.

Description

Description

Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum™ Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum™ automatic 'hot-start' technology. Features of Platinum™ Taq DNA Polymerase, High Fidelity:

Fidelity—greater than six times higher fidelity than Taq DNA polymerase
Amplicon size—amplification of fragments up to 15 kb (see figure)
Convenience—room temperature reaction assembly
Applications—amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR

Unit definition
One unit of Platinum™ Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

Order Info

Shipping Condition: Dry ice

Specifications

Specifications

• Platinum Taq DNA Polymerase High Fidelity (1 x 1000 μL at 5 U/μL)
• 10X High Fidelity Buffer (1 x 50 mL)
• 50 mM MgSO4 (1 x 25 mL)

Store at -10°C to -30°C.
Low
Platinum Taq DNA Polymerase High Fidelity
DNA Polymerase
Hot-start PCR, High-fidelity PCR
Built-In Hot Start
Mixed, 3'-A
20 kb or less
Primer-probe
1-step RT-qPCR
Standard
Dry Ice
6X
5000 Reactions
Separate Components
SDS
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For Research Use Only. Not for use in diagnostic procedures.