
Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.




Measurement | Absorbance at 450 nm minus absorbance (at 550 nm) |
---|---|
Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
Target Species | Human |
Conjugate | HRP |
Description | Human IL-10 ELISA Kit |
Interassay CV | <10% |
Intraassay CV | <10 |
Gene Symbol | CSIF,GVHDS,IL-10 A,TGIF,IL10 |
Total Assay Time | 3 hrs |
Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL |
Assay Sensitivity | <3 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 15.36 to 600 pg/mL |
Test Time | 1 hrs 20 mins |
Accession Number | P22301 |
Regulatory Status | RUO |
Target | IL-10 |
Specificity | No significant cross-reaction with human IL-1 A, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10 |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
Precision | Intra-assay CV <10%; inter-assay CV <10% |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA |
Gene ID (Entrez) | 3586 |
Sample Type | Plasma,Serum,Supernatant,Urine |
Label or Dye | HRP |
Shipping Condition | Wet Ice |
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Content And Storage | 2°C to 8°C |
Host Species | Human |
Conjugate | HRP |
Interassay CV | <10% |
Intraassay CV | <10 |
Total Assay Time | 1 hrs 30 mins |
Sample Volume | Plasma 5 μL, Serum 5 μL |
Protein | SARS-CoV-2 Spike Protein RBD |
Test Time | 40 mins |
Regulatory Status | RUO |
Target | SARS-CoV-2 Spike Protein RBD |
Kit Contents | Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA |
Sample Type | Plasma,Serum |
Invitrogen™ TMB Chromogen Solution (for ELISA)
May be used as a substrate for horseradish peroxidase (HRP) in ELISAs
Invitrogen™ ProcartaPlex™ Porcine Cytokine & Chemokine Panel 1, 9plex
The ProcartaPlex Porcine Cytokine & Chemokine Panel 1 9plex enables the study of immune response by analyzing 9 protein targets in a single well using Luminex xMAP technology.
Invitrogen™ Human MDC ProQuantum Immunoassay Kit
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ELISA
Immunoassay Kit Format | Proximity-based Immunoassay |
---|---|
Target Species | Human |
Conjugate | FAM™ |
Incubation Time | 1 hr. |
Total Assay Time | 2 hrs |
Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
Assay Sensitivity | <1.5 pg/mL |
Storage Requirements | Frozen at -20°C |
Assay Range | 1.6 to 25,000 pg/mL |
For Use With (Application) | Protein Quantitation |
Test Time | 30 mins |
Detection Method | Taqman™ Real-time PCR |
Accession Number | O00626 |
Regulatory Status | RUO |
Target | MDC |
Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
For Use With (Equipment) | qPCR Instruments |
Product Type | ProQuantum |
Gene ID (Entrez) | 6367 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | FAM™ |
Invitrogen™ Human SAA ProQuantum Immunoassay Kit
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ELISA
Immunoassay Kit Format | Proximity-based Immunoassay |
---|---|
Target Species | Human |
Conjugate | FAM™ |
Incubation Time | 1 hr. |
Total Assay Time | 2 hrs |
Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
Assay Sensitivity | <240 pg/mL |
Storage Requirements | Frozen at -20°C |
Assay Range | 256 to 4,000,000 pg/mL |
For Use With (Application) | Protein Quantitation |
Test Time | 30 mins |
Detection Method | Taqman™ Real-time PCR |
Accession Number | P0DJI8 |
Regulatory Status | RUO |
Target | SAA |
Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
For Use With (Equipment) | qPCR Instruments |
Product Type | ProQuantum |
Gene ID (Entrez) | 6288 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | FAM™ |
Invitrogen™ Human Adiponectin ProQuantum Immunoassay Kit
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ELISA
Immunoassay Kit Format | Proximity-based Immunoassay |
---|---|
Target Species | Human |
Conjugate | FAM™ |
Incubation Time | 1 hr. |
Total Assay Time | 2 hrs |
Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
Assay Sensitivity | <0.7 pg/mL |
Storage Requirements | Frozen at -20°C |
Assay Range | 0.76 to 1,500,000 pg/mL |
For Use With (Application) | Protein Quantitation |
Test Time | 30 mins |
Detection Method | Taqman™ Real-time PCR |
Accession Number | Q15848 |
Regulatory Status | RUO |
Target | Adiponectin |
Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
For Use With (Equipment) | qPCR Instruments |
Product Type | ProQuantum |
Gene ID (Entrez) | 9370 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | FAM™ |
Invitrogen™ Human TNF beta ProQuantum Immunoassay Kit
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ELISA
Immunoassay Kit Format | Proximity-based Immunoassay |
---|---|
Target Species | Human |
Conjugate | FAM™ |
Incubation Time | 1 hr. |
Total Assay Time | 2 hrs |
Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
Assay Sensitivity | <0.05 pg/mL |
Storage Requirements | Frozen at -20°C |
Assay Range | 0.064 to 25000 pg/mL |
For Use With (Application) | Protein Quantitation |
Test Time | 30 mins |
Detection Method | Taqman™ Real-time PCR |
Accession Number | P01374 |
Regulatory Status | RUO |
Target | TNF beta |
Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
For Use With (Equipment) | qPCR Instruments |
Product Type | ProQuantum |
Gene ID (Entrez) | 4049 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | FAM™ |
Invitrogen™ Mouse MCP-1 ProQuantum Immunoassay Kit
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ELISA
Immunoassay Kit Format | Proximity-based Immunoassay |
---|---|
Target Species | Mouse |
Conjugate | FAM™ |
Incubation Time | 1 hr. |
Total Assay Time | 2 hrs |
Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
Assay Sensitivity | 0.06 pg/mL |
Storage Requirements | Frozen at -20°C |
Assay Range | 0.064 to 5000 pg/mL |
For Use With (Application) | Protein Quantitation |
Test Time | 30 mins |
Detection Method | Taqman™ Real-time PCR |
Accession Number | P10148 |
Regulatory Status | RUO |
Target | CCL2 (MCP-1) |
Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
For Use With (Equipment) | qPCR Instruments |
Product Type | ProQuantum |
Gene ID (Entrez) | 20296 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | FAM™ |
Target Species | Human |
---|---|
Conjugate | HRP |
Gene Symbol | AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,MPPH,MPPH2,PKB-GAMMA,PKBG,PRKBG,RAC-Pk.-gamma,RAC-gamma,STK-2 |
Total Assay Time | 1 hrs 10 mins |
Sample Volume | Cell Lysate 20 μL |
Storage Requirements | 2°C to 8°C |
Test Time | 1 hrs 20 mins |
Accession Number | P31749 |
Regulatory Status | RUO |
Target | AKT1 |
Kit Contents | Capture Antibody,Detection Antibody Reagent,InstantOne™ Assay Plate,Cell Lysis Buffer,Enhancer Solution,Wash Buffer (10X),Substrate Reagent (TMB),Stop Solution,Positive Control Cell Lysate (lyophilized),Plate seal |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA |
Gene ID (Entrez) | 207 |
Sample Type | Cell Lysates |
Label or Dye | HRP |
For Use With (Equipment) | Colorimetric Microplate Reader |
---|---|
Gene ID (Entrez) | 9332 |
Assay Sensitivity | μg/mL |
Target Species | Rat |
---|---|
Conjugate | HRP |
Total Assay Time | 24 hrs 30 mins |
Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 50 μL |
Assay Sensitivity | 16 ng/ml |
Storage Requirements | 2°C to 8°C |
Assay Range | 16 to 1,000 ng/mL |
Test Time | 1 hrs 45 mins |
Regulatory Status | RUO |
Target | Rat IgA |
Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
For Use With (Equipment) | Colorimetric Microplate Reader |
---|---|
Gene ID (Entrez) | 299357 |
Assay Sensitivity | 0.3125 ng/ml |
Assay Range | 0.3125 to 20 ng/mL |
Target Species | Rat |
---|---|
Conjugate | HRP |
Total Assay Time | 24 hrs 30 mins |
Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 50 μL |
Assay Sensitivity | 6.25 ng/ml |
Storage Requirements | 2°C to 8°C |
Assay Range | 6.25 to 400 ng/mL |
Test Time | 1 hrs 45 mins |
Regulatory Status | RUO |
Target | Rat IgG1 |
Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Human IgG4 protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |