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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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Keyword Search:
1 – 15 of 933 results
1

TSKgel™ Butyl-NPR HPLC Column, 2.5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ Butyl-NPR columns are not as hydrophobic, i.e., less retentive, than Phenyl-5PW and Ether-5PW HIC columns. They are the best choice for high speed separations and provide excellent recovery even for more hydrophobic proteins.

2

Thermo Scientific™ Syncronis™ C18 Columns

Use for hydrophobic interaction reversed-phase separations.

Pore Size 100 Å
Carbon Load 16%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 9
Surface Area 320 m²/g
USP Type L1
3

MilliporeSigma Ascentis™ Express PCS-C18 HPLC Column

Ascentis™ Express PCS-C18 columns are specifically engineered to provide efficient separation of basic, acidic, or neutral compounds under low ionic strength (formic acid) mobile phase conditions.

Pore Size 90 Å
Particle Size 2.7 μm
Type HPLC Column
Phase Positively Charged Surface (PCS)
Material Stainless Steel
Technique HPLC
4

Thermo Scientific™ Acclaim™ C30 HPLC Columns
GREENER_CHOICE

Use for superior separation of hydrophobic structural isomers.

Pore Size 200 Å
Carbon Load 13%
Stationary Phase C30
Temperature 60°C
Phase Reversed Phase
Column Type Reversed Phase
pH 2 to 8
Surface Area 200 m²/g
Product Line Acclaim
5

Restek™ Pinnacle™ DB C8 Specialty/Application Specific HPLC Column, 5 μm  DFS Item

Base-deactivated spherical silica optimized for UHPLC stability.

Pore Size 140 Å
Stationary Phase C8
Temperature 80°C
Phase Reversed Phase
For Use With Analysis of Basic Compounds or Bases Mixed with Acids/Neutrals
Endcapped Yes
Column Type Specialty/Application Specific
Quantity 5 g
Material Stainless Steel
Max. Pressure 4000 psi (275 bar)
Particle Size 5 μm
Ligand Type Monomeric C8
Carbon Load 6%
Packing Material Silica
pH 2.5 to 10
Technique UHPLC
Particle Shape Spherical
Film Thickness 5 μm
USP Type L7
6

Restek™ Viva™ HPLC Columns with C18 Column Packing, 5μm Particle Size  DFS Item

Wide bore silica columns excellent for general-purpose applications and separating peptides or proteins

Pore Size 300 Å
Stationary Phase C18
Temperature 80°C
Phase Reversed Phase
For Use With Analyzing Large Molecules and Biomolecules
Endcapped Yes
Column Type Reversed Phase
Particle Size 5 μm
Type Column
Carbon Load 9%
Packing Material Silica
pH 2.5 to 10
Particle Shape Spherical
Film Thickness 5 μm
USP Type L1
7

Thermo Scientific™ ProPac™ PA1 HPLC Columns

Efficiently analyze and purify hydrophilic anionic proteins and peptides with ProPac™ PA1 LC Columns.

Pore Size Nonporous
Particle Size 10 μm
Flow Rate 0.9 to 1.5 mL/min
Stationary Phase Alkyl Quaternary Amine
Phase Ion Exchange
For Use With BioLC Chromatography System
Packing Material Spherical, Nonporous Polymer
Column Type Ion Exchange
Quantity Each
Green Features FSC
Material PEEK
Max. Pressure 5000 psi (345 bar)
Product Line ProPac
8

Thermo Scientific™ ProPac™ IMAC-10 HPLC Columns
GREENER_CHOICE

Resolve target proteins using a single column in a high-resolution gradient run with ProPac IMAC-10 LC Columns.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Lysozyne Poly(IDA) Grafts
Temperature 60°C
Phase Affinity Separation
Packing Material Spherical, Nonporous Polymer
Column Type Separation of Metal-Binding Proteins
pH 2 to 12
Max. Pressure 3000 psi (207 bar)
9

Thermo Scientific™ ProPac™ WCX-10 HPLC Columns
GREENER_CHOICE

Separate proteins and their variants using these weak cation-exchange columns with exceptionally high resolution.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Carboxylate
Temperature 60°C
Phase Ion Exchange
Column Type Ion Exchange
pH 2 to 12
Quantity Each
Product Line ProPac
10

Thermo Scientific™ ProPac™ WAX-10 HPLC Columns
GREENER_CHOICE

For exceptionally high resolution and efficiency when separating protein variants, use ProPac WAX-10 LC Columns.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Tertiary Amine
Temperature 60°C
Phase Ion Exchange
Packing Material Spherical, Nonporous Polymer
Column Type Ion Exchange
pH 2 to 12
Product Line ProPac
11

Thermo Scientific™ ProSwift™ RP-2H PSDVB Reversed Phase HPLC Column, Monolith, 4.6 mm x 50 mm
GREENER_CHOICE

Column Format: Analytical Column; Pore Size: Monolith; Column Phase: Reversed Phase

12

Thermo Scientific™ ProSwift™ RP-3U PSDVB Reversed Phase HPLC Column, Monolith, 4.6 mm x 50 mm
GREENER_CHOICE

Column Format: Analytical Column; Pore Size: Monolith; Column Phase: Reversed Phase

13

Thermo Scientific™ ProSwift™ SCX-1S Sulfonic Acid Ion Exchange HPLC Column, Monolith, 4.6 mm x 50 mm
GREENER_CHOICE

Column Format: Analytical Column; Pore Size: Monolith; Column Phase: Ion Exchange

14

Thermo Scientific™ Acclaim™ Carbamate HPLC Columns
GREENER_CHOICE

Achieve baseline separation of carbamate specified in U.S. EPA Method 531.2 with high-efficiency Thermo Scientific™ Acclaim™ Carbamate LC Columns.

Pore Size 120 Å
Stationary Phase Proprietary
Temperature 60°C
Endcapped Yes
Column Type Specialty/Application Specific
pH 3 to 7
Surface Area 300 m²/g
Product Line Acclaim
15

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns
GREENER_CHOICE

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Pore Size 120 Å
Carbon Load 8%
Stationary Phase Proprietary
Temperature 70°C
Phase HILIC
For Use With LC/MS
Endcapped Yes
Column Type HILIC
pH 2 to 8
Surface Area 300 m²/g
Product Line Acclaim