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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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Keyword Search:
1 – 15 of 931 results
1

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

2

Thermo Scientific™ Hypersil GOLD™ aQ C18 Polar Endcapped HPLC Columns

Retain and resolve polar analytes with highly aqueous mobile phases.

Pore Size 175 Å
Carbon Load 12%
Temperature 60°C
Phase Reversed Phase
Endcapped Polar
pH 2 to 9
Surface Area 220 m²/g
USP Type L1
3

MilliporeSigma™ Supelco™ Ascentis™ C18 HPLC Column, 3 μm Particle Size

Ascentis™ C18 is an extremely stable and reliable HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Pore Size 100 Å
Particle Size 3 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 8
Technique Fused-Core HPLC
Surface Area 450 m2/g
Product Line Ascentis
4

MilliporeSigma™ Supelco™ Kromasil C8 HPLC Column, 100 Å, 5 μm Particle Size

For small molecules and peptides.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase
Product Line Kromasil
5

Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns

Use for enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.

6

Thermo Scientific™ Acclaim™ Surfactant Plus HPLC Columns
GREENER_CHOICE

Use for simultaneous separation of anionic, nonionic, cationic, and amphoteric surfactants with CAD and LC-MS detection.

Pore Size 120 Å
Stationary Phase Proprietary
Temperature 50°C
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Specialty/Application Specific
pH 2.5 to 7.5
Green Features FSC
Surface Area 300 m²/g
Product Line Acclaim
7

Thermo Scientific™ Acclaim™ Surfactant HPLC Columns
GREENER_CHOICE

Use for outstanding separation of anionic, nonionic, and cationic surfactants with ELSD and RI detectors.

Pore Size 120 Å
Carbon Load 12%
Stationary Phase Proprietary
Temperature 60°C
Endcapped Yes
Column Type Specialty/Application Specific
pH 2.5 to 7.5
Green Features FSC
Surface Area 300 m²/g
Product Line Acclaim
8

Thermo Scientific™ BioBasic™ 4 HPLC Columns

Use this 300Å wide pore silica for analysis of larger hydrophobic proteins and peptides that bind too tightly on C8 or C18 columns.

Pore Size 300 Å
Particle Size 5 μm
Carbon Load 4%
Stationary Phase C4
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 9
Surface Area 100 m²/g
Max. Pressure 5800 psi (400 bar)
USP Type L26
9

Thermo Scientific™ Hypersil GOLD™ Phenyl HPLC Columns

Use for quick analysis of aromatic analytes, including glucocorticosteroids, estrogens, fat-soluble vitamins, and phospholipids.

Pore Size 175 Å
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
pH 2 to 8
Surface Area 220 m²/g
USP Type L11
10

Thermo Scientific™ Syncronis™ C8 HPLC Columns

Use this C8 column for hydrophobic interaction reversed-phase separations.

Pore Size 100 Å
Carbon Load 10%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 320 m²/g
USP Type L7
11

Thermo Scientific™ Hypersil™ Phenyl-BDS HPLC Columns

Use as a robust, general-purpose column with aromatic selectivity for QC/QA labs.

Pore Size 130 Å
Carbon Load 5%
Stationary Phase Phenyl (Ph)
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Base-Deactivated Silica
Column Type Reversed Phase
Surface Area 170 m²/g
Max. Pressure 5800 psi (400 bar)
USP Type L11
12

Thermo Scientific™ Acclaim™ Polar Advantage II HPLC Columns
GREENER_CHOICE

Resolve polar and nonpolar compounds in a single run. These columns have high pH stability (pH 1.5–10) in the separation of basic, neutral, and acidic species.

Pore Size 120 Å
Stationary Phase Polar Embedded
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
Surface Area 300 m²/g
USP Type L60
13

Thermo Scientific™ Hypersil GOLD™ C8 HPLC Columns

Have analytes with medium hydrophobicity or need a less hydrophobic phase to obtain optimum retention? These columns provide outstanding sensitivity and excellent peak shape.

Pore Size 175 Å
Carbon Load 8%
Stationary Phase C8
Temperature 60°C
pH 2 to 9
Surface Area 220 m²/g
USP Type L7
14
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Thermo Scientific™ Hypersil™ ODS-2 C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes separated under difficult operating conditions.

Pore Size 80 Å
Carbon Load 11%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous Type A Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 220 m²/g
Max. Pressure 5800 psi (400 bar)
Product Line Hypersil Classical
USP Type L1
15

Thermo Scientific™ Syncronis™ Phenyl HPLC Columns

Use this high-surface-area column for aromatic compound separations.

Pore Size 100 Å
Carbon Load 11%
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 320 m²/g
USP Type L11