HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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Thermo Scientific™ ProPac™ PA1 HPLC Columns

Efficiently analyze and purify hydrophilic anionic proteins and peptides with ProPac™ PA1 LC Columns.

Pricing and Availability
Specifications

Pore Size	Nonporous
Particle Size	10 μm
Flow Rate	0.9 to 1.5 mL/min
Stationary Phase	Alkyl Quaternary Amine
Phase	Ion Exchange
For Use With	BioLC Chromatography System
Packing Material	Spherical, Nonporous Polymer
Column Type	Ion Exchange
Quantity	Each
Green Features	FSC
Material	PEEK
Max. Pressure	5000 psi (345 bar)
Product Line	ProPac

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Pricing and Availability
Specifications

Pore Size	120 Å
Carbon Load	8%
Stationary Phase	Proprietary
Temperature	70°C
Phase	HILIC
For Use With	LC/MS
Endcapped	Yes
Column Type	HILIC
pH	2 to 8
Surface Area	300 m²/g
Product Line	Acclaim

MilliporeSigma™ Supelco™ Proteomix™ SCX-NP3 Strong Cation Exchange Column, 3 μm Particle Size, PEEK

Used for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.

Pricing and Availability
Specifications

Pore Size	Nonporous
Particle Size	3 μm
Stationary Phase	Sulfonic Acid
Phase	Cation Exchange
Matrix	Spherical PS-DVB/Sulfonic Acid Phase
Length (Metric)	5 cm
pH	2.0 to 12.0
Material	PEEK
Technique	Cation Exchange
Product Line	Proteomix

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 10 μm Particle Size

The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	10 μm
Stationary Phase	C18 (octadecyl)
Phase	Reversed Phase
Matrix	C18 (octadecyl) Phase/silica particle platform
Endcapped	Yes
Technique	Wide Pore HPLC
Surface Area	100 m2/g
Product Line	Discovery

MilliporeSigma™ Supelco™ Carboxen™ 569 Thermal Desorption Tubes

Suitable for use with Air Monitoring

Pricing and Availability
Specifications

Type	Thermal Desorption Tube
Matrix	Carboxen™ 569 Carbon Molecular Sieve (CMS)
For Use With (Equipment)	PerkinElmer, Markes, DANI, OI Analytical, and Shimadzu Instruments
Diameter (English) Outer	1/4 in.
Packaging Quantity	10/Pk.
Material	Glass
Overall Length (English)	3.5 in.
For Use With (Application)	Air Monitoring
Product Line	Carboxen

MilliporeSigma™ Supelco™ Antibodix™ WCX-NP10 Weak Cation Exchange Column, 10 μm Particle Size, PEEK

Antibodix™ columns are designed for the reproducible analysis of charge variants of monoclonal antibodies by salt or pH gradient.

Pricing and Availability
Specifications

Pore Size	Nonporous
Particle Size	10 μm
Stationary Phase	Spherical PS-DVB
Phase	Cation Exchange
Matrix	Spherical PS-DVB Phase
pH	2.0 to 12.0
Material	PEEK
Technique	Cation Exchange
Product Line	Antibodix

MilliporeSigma™ Supelco™ SRT™ SEC-500 Gel Filtration Column, 5 μm Particle Size, PEEK

Sepax SRT™ SEC columns feature a hydrophilic surface layer that effectively shields the highly porous, five micron silica particles from interacting with your sample.

Pricing and Availability

MilliporeSigma™ Supelco™ SRT™ SEC-150 Gel Filtration Column, 5 μm Particle Size, PEEK

Sepax SRT™ SEC columns feature a hydrophilic surface layer that effectively shields the highly porous, five micron silica particles from interacting with your sample.

Pricing and Availability

Thermo Scientific™ BetaBasic™ 18 HPLC Columns, 5μm Particle Size

Available in limited dimensions. Please contact Customer Service for more details.

Pricing and Availability
Specifications

Pore Size	150 Å
Particle Size	5 μm
Carbon Load	13%
Stationary Phase	C18
Phase	Reversed Phase
For Use With	LC/MS, APCI
Endcapped	Yes
Packing Material	Silica
Column Type	Reversed Phase
pH	2 to 12
Surface Area	200 m²/g
USP Type	L1
Column Format	Analytical Column

Thermo Scientific™ POROS™ CaptureSelect™ FSH Affinity HPLC Columns

Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates.

Pricing and Availability
Specifications

Pore Size	2000 Å
Content And Storage	Column includes EZ grip fittings and column test certificate Store at 4 °C. DO NOT FREEZE.
Stationary Phase	Follicle Stimulating Hormone affinity ligand, humanspecific
Column Type	Affinity
Quantity	1 Column
Material	PEEK
For Use With (Application)	FPLC
High-throughput Compatibility	Automated Protocols
Max. Pressure	180 bar
Particle Size	20 μm
Type	Column
Target Molecule	Follicle Stimulating Hormone
Binding Capacity	1.5 mg/mL resin
Sample Type	Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures
Purification Target	Follicle Stimulating Hormone
Product Line	CaptureSelect, POROS

MilliporeSigma™ Supelco™ SRT™-C SEC-300 Gel Filtration Column, 5 μm Particle Size, Stainless Steel

The bonded phase in SRT™-C SEC columns is more polar than the hydrophilic phase used in SRT™ columns, making SRT™-C columns better suited for the gel filtration analysis of more hydrophobic bioopolymers such as membrane proteins and pegylated antibodies.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Stationary Phase	Hydrophilic Polymer
Phase	Size Exclusion
Matrix	Hydrophilic Polymer Phase/Spherical Silica
Length (Metric)	30 cm
pH	2.0 to 8.5
Material	Stainless Steel
Technique	SEC HPLC
Product Line	SRT

MilliporeSigma™ Supelco™ SRT™-C SEC-1000 Gel Filtration Column, 5 μm Particle Size, Stainless Steel

Pricing and Availability
Specifications

Pore Size	1000 Å
Particle Size	5 μm
Stationary Phase	Hydrophilic Polymer
Phase	Size Exclusion
Matrix	Hydrophilic Polymer Phase/Spherical Silica
Length (Metric)	30 cm
pH	2.0 to 8.5
Material	Stainless Steel
Technique	SEC HPLC
Product Line	SRT

MilliporeSigma™ Supelco™ Unix™ SEC-300 UHPLC Column, 1.8 μm Particle Size

Provides high stability and negligible non-specific interactions.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	1.8 μm
Stationary Phase	Neutral, Hydrophilic Film Bonded to Silica
Phase	Size Exclusion
Matrix	Neutral, Hydrophilic Film Bonded to Silica Phase/Spherical Silica
Diameter (Metric)	4.6 mm
Technique	SEC UHPLC
Product Line	Unix

Thermo Scientific™ Hypersil™ PREP HS C18 HPLC Columns

Maximize retention and loading with Hypersil PREP HS C18 prep LC reversed phase columns which offers high loading capacity and suitable for USP-L1 Applications.

Pricing and Availability
Specifications

Pore Size	100 Å
Carbon Load	15%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Column Type	Reversed Phase
pH	2 to 9
Surface Area	350 m²/g
USP Type	L1

TSKgel™ CN-80Ts HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Comprised of cyanopropyl groups bonded to the silica. Applications include reversed-phase high performance liquid chromatographic techniques.

Pricing and Availability
Specifications

Shipping Condition	Shipped in 60% Acetonitrile, 40% Water
Pore Size	80 Å
Solvents	0-100% Organic Solvent
Stationary Phase	Cyano
Temperature	10°C to 50°C
Phase	Reversed Phase
Endcapped	Yes
Material	Stainless Steel Hardware
Surface Area	425 m²/g
Particle Size	5 μm
Flow Rate	1.2 mL/min
Carbon Load	8%
Matrix	Cyano Phase/Silica Particle Platform
Diameter (Metric)	4.6 mm
pH	2 to 7.5
Technique	Reverse Phase HPLC
Product Line	TSKgel
USP Type	L10

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Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns