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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 933 results
1

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C5 HPLC Column, 10 μm Particle Size

Discovery™ BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC.

Pore Size 300 Å
Particle Size 10 μm
Stationary Phase C5 (pentyl)
Phase Reversed Phase
Matrix C5 (pentyl) Phase/Silica Particle Platform
Endcapped Yes
Technique Wide Pore HPLC
Surface Area 100 m2/g
Product Line Discovery
2

Thermo Scientific™ Syncronis™ C18 Columns

Use for hydrophobic interaction reversed-phase separations.

Pore Size 100 Å
Carbon Load 16%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 9
Surface Area 320 m²/g
USP Type L1
3

TSKgel™ Butyl-NPR HPLC Column, 2.5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ Butyl-NPR columns are not as hydrophobic, i.e., less retentive, than Phenyl-5PW and Ether-5PW HIC columns. They are the best choice for high speed separations and provide excellent recovery even for more hydrophobic proteins.

4

MilliporeSigma Ascentis™ Express PCS-C18 HPLC Column

Ascentis™ Express PCS-C18 columns are specifically engineered to provide efficient separation of basic, acidic, or neutral compounds under low ionic strength (formic acid) mobile phase conditions.

Pore Size 90 Å
Particle Size 2.7 μm
Type HPLC Column
Phase Positively Charged Surface (PCS)
Material Stainless Steel
Technique HPLC
5

MilliporeSigma™ Supelco™ Discovery™ C18 HPLC Column, 5 μm Particle Size

Use Discovery™ C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.

Pore Size 180 Å
Particle Size 5 μm
Stationary Phase C18 (Octadecyl)
Phase Reversed Phase
Matrix C18 (Octadecyl) Phase/Silica Gel, High Purity, Spherical Particle Platform
Endcapped Yes
pH 2 to 8
Material Stainless Steel
Surface Area 200 m2/g
Product Line Discovery
6

Thermo Scientific™ Acclaim™ C30 HPLC Columns

Use for superior separation of hydrophobic structural isomers.

Pore Size 200 Å
Carbon Load 13%
Stationary Phase C30
Temperature 60°C
Phase Reversed Phase
Column Type Reversed Phase
pH 2 to 8
Surface Area 200 m²/g
Product Line Acclaim
7

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 10 μm Particle Size

The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Pore Size 300 Å
Particle Size 10 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/silica particle platform
Endcapped Yes
Technique Wide Pore HPLC
Surface Area 100 m2/g
Product Line Discovery
8

Thermo Scientific™ Accucore™ 150-C4 HPLC Columns

Use this specially designed solid core column with a C4 functionality for biomolecules that bind too tightly to C8 or C18 columns.

Pore Size 150 Å
Particle Size 2.6 μm
Carbon Load 2%
Stationary Phase C4
Temperature 60°C
Phase Reversed Phase
For Use With HPLC
Endcapped Yes
Packing Material Spherical, Solid Core, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 9
Surface Area 80 m²/g
USP Type L26
9

Restek™ Pinnacle™ DB C8 Specialty/Application Specific HPLC Column, 5 μm  DFS Item

Base-deactivated spherical silica optimized for UHPLC stability.

Pore Size 140 Å
Stationary Phase C8
Temperature 80°C
Phase Reversed Phase
For Use With Analysis of Basic Compounds or Bases Mixed with Acids/Neutrals
Endcapped Yes
Column Type Specialty/Application Specific
Quantity 5 g
Material Stainless Steel
Max. Pressure 4000 psi (275 bar)
Particle Size 5 μm
Ligand Type Monomeric C8
Carbon Load 6%
Packing Material Silica
pH 2.5 to 10
Technique UHPLC
Particle Shape Spherical
Film Thickness 5 μm
USP Type L7
10

Restek™ Viva™ HPLC Columns with C18 Column Packing, 5μm Particle Size  DFS Item

Wide bore silica columns excellent for general-purpose applications and separating peptides or proteins

Pore Size 300 Å
Stationary Phase C18
Temperature 80°C
Phase Reversed Phase
For Use With Analyzing Large Molecules and Biomolecules
Endcapped Yes
Column Type Reversed Phase
Particle Size 5 μm
Type Column
Carbon Load 9%
Packing Material Silica
pH 2.5 to 10
Particle Shape Spherical
Film Thickness 5 μm
USP Type L1
11

Hamilton™ PRP-1 Reversed Phase HPLC Columns, 10um Particle Size: PEEK

Stable alternative to C8 or C18 Columns

Pore Size 100 Å
Stationary Phase PRP-1
Temperature 5°C to 85°C
Phase Reversed Phase
Column Type Reversed Phase
Material PEEK
Max. Pressure 5000 psi (345 bar)
Column Format Analytical Column
Particle Size 10 μm
Flow Rate 1.0 mL/min
Type Column
Packing Material PSDVB (Polystyrene Divinylbenzene)
Mobile Phase Compatibility pH 1 to 13, 0 to 100% aqueous, organic modifier
Diameter (Metric) 4.6 mm
pH 1 to 13
USP Type L21
12

Hamilton™ PRP™-3 Reversed Phase HPLC Columns

PRP-3 polymeric 300 Å columns for gradient separations of peptides and proteins

Pore Size 300 Å
Particle Size 10 μm
Stationary Phase PRP-3
Temperature 5°C to 85°C
Phase Reversed Phase
Packing Material PSDVB (Polystyrene Divinylbenzene)
Column Type Reversed Phase
pH 1 to 13
Max. Pressure 5000 psi (345 bar)
USP Type L21
13

Hamilton™ PRP-1 Reversed Phase HPLC Columns, 10um Particle Size: Stainless Steel

Stable alternative to C8 or C18 Columns

Pore Size 100 Å
Type Column
Stationary Phase PRP-1
Temperature 5°C to 85°C
Phase Reversed Phase
Packing Material PSDVB (Polystyrene Divinylbenzene)
Column Type Reversed Phase
Mobile Phase Compatibility pH 1 to 13, 0 to 100% aqueous, organic modifier
pH 1 to 13
Material Stainless Steel
Max. Pressure 5000 psi (345 bar)
USP Type L21
Column Format Analytical Column
14

MilliporeSigma™ LiChroCART™ Column/LiChrospher™ 100 RP-18 Endcapped Sorbent HPLC Cartridges, 5μm

HPLC cartridge

Pore Size 100 Å
Type Cartridge
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
Material Stainless Steel
USP Type L1
15

Thermo Scientific™ POROS™ HS 20 μm Strong Cation Exchange HPLC Columns
GREENER_CHOICE

Thermo Scientific™ POROS™ Prepacked Strong Cation Exchange Column. POROS HS is a high capacity, strong cation exchange, High Performance Chromatography™ resins suitable for the separation and purification of biomolecules.

Shipping Condition Room Temperature
Pore Size 2000 Å
Particle Size 20 μm
Type Prepacked Column
Content And Storage Store at room temperature.
Stationary Phase Sulfopropyl Polymer
Binding Capacity 75 mg/mL (Lysozyme pH 6.2)
Column Type Ion Exchange
Quantity 1 Column
Material PEEK
Product Line POROS