Search results for "STEMCELL Technologies"
Stemcell Technologies Inc mTeSR™1
Use this specialized, feeder-free culture medium to achieve more consistent human pluripotent stem cell cultures with homogenous, undifferentiated phenotypes. Manufactured under relevant cGMPs, mTeSR™1 ensures the highest quality and consistency for reproducible results in your fundamental research, as well as for cell therapy and investigational new drug research applications. This serum-free, complete cell culture medium is made with pre-screened raw materials to ensure batch-to-batch consistency and robust performance in feeder-free hPSC culture. Use established protocols for applications ranging from derivation to differentiation with this most widely published feeder-free hPSC culture medium, which has been used by leading pluripotent stem cell researchers to successfully maintain thousands of hPSC lines in over 50 countries.
Stemcell Technologies Inc 100 mm Dish, Tissue Culture-Treated, Size: 20 Dishes
Tissue Culture-Treated Dishes are ideal for attachment-dependent cell and tissue culture. These polystyrene dishes are transparent for superior optical clarity and are stackable for easy handling and storage. The dishes have been sterilized and are vented to allow for gas exchange. 20 dishes/pk
Stemcell Technologies Inc CRYOSTOR CSB, SIZE 100 ML
CryoStor CSB is an intracellular-like base formulation from the CryoStor preservation media series. It is serum-free, animal component-free, and does not contain dimethyl sulfoxide (DMSO). It is designed to improve post-thaw cell viability and function, and to reduce the level of cryopreservation-induced delayed-onset cell death.
Stemcell Technologies Inc SepMate™-50 (IVD)
Simplify peripheral blood mononuclear cell (PBMC) isolation by incorporating SepMate™ into your density gradient centrifugation step. SepMate™ tubes contain an insert that creates a barrier between the density gradient medium and blood, thus eliminating the need for careful blood layering and allowing mononuclear cells to be easily harvested with a simple pour. This product can be used with RosetteSep™ to isolate specific immune cell subsets.SepMate™ is manufactured under cGMP and registered as an In Vitro Diagnostic (IVD) device in Australia, Canada, Europe, and the USA. In China, SepMate™ is considered a nonmedical device by the China Food and Drug Administration (CFDA) and should be used as general lab equipment. The end user is responsible for determining whether the product is suitable for their specific application.Browse our Frequently Asked Questions (FAQs) (https://www.stemcell.com/sepmate-faq.html) on SepMate™.
Stemcell Technologies Inc STEMdiff™ Cardiomyocyte Dissociation Kit, Size: 1 Kit
STEMdiff™ Cardiomyocyte Dissociation Kit includes STEMdiff™ Cardiomyocyte Dissociation Medium and STEMdiff™ Cardiomyocyte Support Medium. STEMdiff™ Cardiomyocyte Dissociation Medium can be used to harvest cardiomyocytes that have been differentiated from human pluripotent stem cells (hPSCs) using STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (Catalog #05010) or STEMdiff™ Atrial Cardiomyocyte Differentiation Kit (Catalog #100-0215) and maintained in STEMdiff™ Cardiomyocyte Maintenance Medium (Catalog #05010/05020). STEMdiff™ Cardiomyocyte Support Medium reduces stress on these cardiomyocytes during harvesting and replating, maintaining their viability and functional capacity for downstream applications and analyses.
Stemcell Technologies Inc hMSC CD45/CD73/CD90/CD105 Antibody Panel
The MSC Characterization Antibody Panel is designed for the phenotypic analysis of human mesenchymal stromal cells (MSCs). This kit contains three fluorochrome-conjugated antibodies for the identification of human MSCs, and a fluorochrome-conjugated antibody for the identification of non-MSCs. When measured by flow cytometry, ≥ 95% of the MSC population must express CD73, CD90, and CD105, and these cells must lack expression (≤ 2% positive) of CD45. These reagents provide single-step labeling for the verification of human MSCs.The MSC Characterization Antibody Panel can be used to identify human MSCs derived from various tissues such as bone marrow MSCs, pluripotent stem cell-derived mesenchymal progenitor cells, adipose-derived MSCs, dental pulp-derived MSCs, and umbilical cord-derived MSCs.
Stemcell Technologies Inc CryoStor CS10, Size: 50 mL
Maximize post-thaw cell recovery and viability following cryopreservation at very low temperatures (-80C to -196C) with ready-to-use CryoStor CS10 medium. Serum-free, animal component-free, and cGMP-manufactured, this defined medium provides a safe, protective environment that is recommended for the cryopreservation of a variety of sensitive cell and sample types, including myeloma cell lines, human pluripotent stem cells, human peripheral blood, and more. Available in a variety of convenient formats, CryoStor CS10 is formulated with USP-grade components to minimize variability and contains 10% DMSO. Visit STEMCELL.com/cryostor-cs10 for more information.
Stemcell Technologies Inc Collagenase Type IV, Size: 100 mg
Collagenase is a protease consisting of a single polypeptide chain approximately 1,000 amino acid residues in length. Collagenase is capable of digesting native collagen fibrils commonly found in connective tissues and therefore is frequently used for tissue dissociation. Collagenase preparations contain the activity of several proteases, including collagenase, caseinase, clostripain, and trypsin (Kessler & Yaron). Collagenase Type IV contains low levels of tryptic activity and is frequently used for pancreatic islets (Taguchi et al.) and for applications where maintenance of receptor integrity is required.
Stemcell Technologies Inc STEMdiff™ APEL™2 Medium, Size: 100 mL
STEMdiff™ APEL™ 2 Medium is a fully defined, serum-free and animal component-free medium for the differentiation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. It is based on the APEL formulation published by Dr. Andrew Elefanty and lacks undefined components such as protein-free hybridoma medium.STEMdiff™ APEL™ 2 can be used in adherent or embryoid body (EB)-based protocols, such as with AggreWell™. It can be used with a variety of different induction factors or cytokines to support differentiation along ectoderm, mesoderm and endoderm lineages.
Stemcell Technologies Inc Anti-Adherence Rinsing Solution, Size: 100 mL
Formerly known as AggreWell™ Rinsing Solution, name change effective Dec 2017.Anti-Adherence Rinsing Solution is a surfactant solution for pre-treating cultureware to reduce surface tension and prevent cell adhesion. It can be used with plates, flasks, pipettes, strainers, and other cultureware. Anti-Adherence Rinsing Solution is required for use with the following: AggreWell™ plates (e.g. Catalog #34811) Organoid growth media, including HepatiCult™ (Catalog #06030) and PancreaCult™ (Catalog #06040)
Stemcell Technologies Inc DNase I Solution (1 mg/mL), Size: 1 mL
Deoxyribonuclease (DNase) I Solution (1 mg/mL) is useful to reduce or prevent the clumping of concentrated and/or cryopreserved cell suspensions following thawing. The activity range is 2000 units per mg of protein.
Stemcell Technologies Inc MyeloCult™ H5100, Size: 500 mL
MyeloCult™ H5100 is a culture medium for the initiation and maintenance of myeloid long-term cultures of human hematopoietic cells and stromal cell feeder layers. The serum used in this formulation has been pre-tested and selected for its ability to support long-term myelopoiesis by primitive human hematopoietic cells (e.g. in long-term culture-initiating cell (LTC-IC) assays). MyeloCult™ H5100 requires the addition of freshly prepared Hydrocortisone (Catalog #74142).
Stemcell Technologies Inc RoboSep™ PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ PE Positive Selection Kit (Catalog #18557) for even faster cell isolations.
Stemcell Technologies Inc Hydrocortisone, Size: 100 mg
Support the growth and differentiation of endothelial, epithelial, mesenchymal, or oligodendrocyte cells with Hydrocortisone. This glucocorticoid hormone, with anti-inflammatory and immunosuppressive effects (Kang et al. Biol Pharm Bull, 2001; Langhoff & Ladefoged. Eur J Clin Pharmacol, 1983), enhances proliferation and self-renewal of limbal stem cells in explant cultures (Yu et al. Exp Eye Res, 2016) and supports long-term growth of immature human myeloid cells in culture (Salahuddin et al. Blood, 1981). Hydrocortisone also promotes differentiation of mouse embryonic stem (ES) cell-derived definitive endoderm toward lung alveolar epithelial cells (Mokhber Dezfouli et al. Cell J, 2019). The product is supplied as the sodium salt of hydrocortisone 21-hemisuccinate, which is a more water-soluble form of hydrocortisone. Hydrocortisone comes with 98% purity; Molecular weight 484.5 g/mol; CAS Number 125-04-2. Visit STEMCELL.com for more information and related products.
Stemcell Technologies Inc RHO/ROCK PATHWAY INHIBITOR IN
RHO/ROCK pathway inhibitor; Inhibits ROCK.