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  5. 5017408

Invitrogen™ Mouse TSLP Uncoated ELISA Kit

Numéro de catalogue. 5017408
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10 x 96 Tests
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Numéro de catalogue. Quantité
5017408 10 x 96 Tests
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Numéro de catalogue. 5017408 Fournisseur Invitrogen™ Code fournisseur 88749088

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Description

ELISA

The Mouse TSLP Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse TSLP. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.

Spécifications

Numéro d'enregistrement Q9JIE6
Plage de l'essai 16-2000 pg/mL
Sensibilité d’essai 16 pg/mL
Contenu et stockage Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards
96-Well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
Conjugué HRP
Format de la trousse d’immunodosage Uncoated ELISA Kit
Type de produit ELISA
Type d’échantillon Serum, Supernatant
Espèces cibles Mouse
À utiliser avec (application) ELISA
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène Thymic stroma-derived lymphopoietin; thymic stromal lymphopoietin; TSLPThymic stroma-derived lymphopoietin; thymic stromal lymphopoietin; TSLP; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3
ID du gène (Entrez) 53603
Symbole de gène TSLP
Contenus des kits Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86), Purified antibody, Detection Antibody: Pre-titrated, Biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
Étiquette ou colorant Biotin
Alias des protéines Thymic stroma-derived lymphopoietin
Quantité 10 x 96 Tests
Statut réglementaire RUO
Volume d’échantillon Serum 100 μL, Supernatant 100 μL
Conditions de stockage 2-8°C
Synonyme Thymic Stromal-Derived Lymphopoietin
Cible TSLP
Durée du test 1 hr 45 min
Durée totale de l'essai 24 hr 30 min
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