Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (4,080)

Singleplex and Multiplex Bead Based Immunoassays (526)

ELISA Substrates and Reagents (82)

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1 – 15 of 3,569 results

Invitrogen™ Mouse HMGB-1 ELISA Kit

ELISA

Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
Protein Family	High mobility group
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	<10%
Intraassay CV	<10%
Gene Symbol	Hmgb1
Total Assay Time	3 hr 30 min
Assay Sensitivity	9.38 pg/mL
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	15.63-1000 pg/mL
Test Time	1 hr 20 min
Accession Number	P63158
Regulatory Status	RUO
Target	HMGB1
Kit Contents	Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer
Gene Alias	Ac2-008; amphoterin; Amphoterin antibody; DEF; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; high mobility group 1 protein; high mobility group box 1; high mobility group protein 1; High mobility group protein B1; high mobility group protein HMG1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; high-mobility-group protein; Hmg1; HMG-1; HMG3; HMG3 antibody; hmgb 1; HMGB1; hmgb-1; HMGB1 protein; hypothetical protein; non-histone protein HMG1; p30; RCJMB04_15a21; RP11-550P23.1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein productAc2-008; amphoterin; Amphoterin antibody; DEF; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; high mobility group 1 protein; high mobility group box 1; high mobility group protein 1; High mobility group protein B1; high mobility group protein HMG1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; high-mobility-group protein; Hmg1; HMG-1; HMG3; HMG3 antibody; hmgb 1; HMGB1; hmgb-1; HMGB1 protein; hypothetical protein; non-histone protein HMG1; p30; RCJMB04_15a21; RP11-550P23.1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein product;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	15289
Sample Type	Plasma,Serum
Label or Dye	Biotin

Invitrogen™ Human VEGF ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.1%
Intraassay CV	4.7%
Incubation Time	4 hr.
Gene Symbol	VEGFA
Total Assay Time	4 hr
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<5 pg/mL
Storage Requirements	2-8°C
Assay Range	23.4-1500 pg/mL
Marker Gene	VEGF
For Use With (Application)	Colorimetric Detection
Test Time	1 hr 20 min
Accession Number	P15692
Regulatory Status	RUO
Target	VEGF
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7422
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Mouse IL-13 Uncoated ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	Il13
Total Assay Time	24 hr 30 min
Sample Volume	Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	8 pg/mL
Storage Requirements	2-8°C
Assay Range	8-500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P20109
Regulatory Status	RUO
Target	IL-13
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, eBioscience ELISA/ ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,eBioscience ELISA/ ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16163
Sample Type	Serum,Supernatant
Label or Dye	Biotin
Synonym	Interleukin-13, IL13

Invitrogen™ Mouse IL-5 Uncoated ELISA Kit

ELISA

Pricing and Availability
Specifications

Immunoassay Kit Format	Uncoated ELISA Kit
Content And Storage	Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, biotin-conjugated antibody Standard: Recombinant protein for generating standard curve and calibrating samples 10 x Coating Buffer: Buffer for plating the Capture Antibody 5 x ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Enzyme: Pre-titrated Avidin-HRP Substrate: 1 x TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96-well Plate: Corning Costar 9018 (included with product Cat. No. ending in suffixes -22, -76, -86)
Target Species	Mouse
Conjugate	HRP
Gene Symbol	Il5
Total Assay Time	24 hr 30 min
Sample Volume	Lavage Fluid 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	8 pg/mL
Storage Requirements	2-8°C
Assay Range	8-500 pg/mL
Test Time	1 hr 45 min
Accession Number	P04401
Regulatory Status	RUO
Target	IL-5
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Gene Alias	B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRFB-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRF; CANDF6; IL-17F; ML-1; ML1
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16191
Sample Type	Lavage Fluid,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human PF4 ELISA Kit

ELISA

Pricing and Availability
Specifications

Protein Family	Chemokines & Receptors
Target Species	Human
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Assay Sensitivity	20 pg/mL
Research Discipline	Inflammation
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	PF4
Specificity	This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Interassay CV	<12%
Gene Symbol	Pf4
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	20.58-15000 pg/mL
Accession Number	P02776
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	5196
Sample Type	Plasma,Serum,Supernatant
Synonym	CXCL4, PF-4, SCYB4

Invitrogen™ Serotonin Competitive ELISA Kit

ELISA

Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	All Species
Conjugate	HRP
Interassay CV	<10%
Intraassay CV	<10%
Total Assay Time	2 hr 30 min
Assay Sensitivity	9.38 ng/mL
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	15.63-1000 ng/mL
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Serotonin
Kit Contents	Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer
Gene Alias	5-HT; 5-hydroxytryptamne5-HT; 5-hydroxytryptamne;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Sample Type	Plasma,Serum
Label or Dye	Biotin

Invitrogen™ Human Cathepsin D ELISA Kit

ELISA

Pricing and Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	Ctsd
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.049 ng/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	0.049-12 ng/mL
Test Time	1 hr 20 min
Accession Number	P07339
Regulatory Status	RUO
Target	Cathepsin D
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	1509
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Cell Extraction Buffer

Suitable for use in ELISA and Western blotting

Pricing and Availability

Invitrogen™ eBioscience™ ProcartaPlex Rat Eotaxin (CCL11) Simplex

Ideal solution for assessing multiple protein biomarkers in a single sample. Explore our broad portfolio of pre-configurated panels and combinable single analytes.

Pricing and Availability

Invitrogen™ eBioscience™ ProcartaPlex Human Eotaxin-3 (CCL26) Simplex

Ideal solution for assessing multiple protein biomarkers in a single sample. Explore our broad portfolio of pre-configurated panels and combinable single analytes.

Pricing and Availability

Invitrogen™ BLC (CXCL13) Human ProcartaPlex™ Simplex Kit

Ideal solution for assessing multiple protein biomarkers in a single sample. Explore our broad portfolio of pre-configurated panels and combinable single analytes.

Pricing and Availability

Human IL-1 alpha ELISA Development Kit (TMB), PeproTech®

ELISA

Pricing and Availability
Specifications

Shipping Condition	Room temperature
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human
Conjugate	HRP
Gene Symbol	Il1a
Total Assay Time	24 hr 30 min
Assay Sensitivity	Lot-specific
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	Lot-specific
Test Time	1 hr 45 min
Accession Number	P01583
Regulatory Status	RUO
Target	IL-1 alpha
Kit Contents	This Human IL-1 alpha ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.,Detection Antibody,Protein Standard,HRP-conjugate
Gene Alias	CTLA8; hematopoietin-1; IL 1 a; IL 1α; IL1; IL-1 alpha; IL17; IL1A; Il-1a; IL-1alpha; IL1-ALPHA; IL1F1; IL1α; ILN; Interleukin; interleukin 1 alpha; interleukin 1, alpha; interleukin 17; interleukin 1a; interleukin 1-alpha; interleukin 1-alpha (AA 1 - 152); Interleukin1 alpha; interleukin-1 alpha; interleukin-1 alpha precursor; Interleukin-17A; preinterleukin 1 alpha; pre-interleukin-1 alpha; pro-interleukin-1-alpha; RP23-160G19.8CTLA8; hematopoietin-1; IL 1 a; IL 1α; IL1; IL-1 alpha; IL17; IL1A; Il-1a; IL-1alpha; IL1-ALPHA; IL1F1; IL1α; ILN; Interleukin; interleukin 1 alpha; interleukin 1, alpha; interleukin 17; interleukin 1a; interleukin 1-alpha; interleukin 1-alpha (AA 1 - 152); Interleukin1 alpha; interleukin-1 alpha; interleukin-1 alpha precursor; Interleukin-17A; preinterleukin 1 alpha; pre-interleukin-1 alpha; pro-interleukin-1-alpha; RP23-160G19.8;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	3552
Label or Dye	Biotin

Invitrogen™ Human SLC (CCL21) Uncoated ELISA Kit with Plates

ELISA

Pricing and Availability

Invitrogen™ Mouse IL-1RA ELISA Kit

ELISA

Pricing and Availability
Specifications

Protein Family	Other Proteins
Target Species	Mouse
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 20 μL, Serum 20 μL, Supernatant 50 μL
Assay Sensitivity	2 pg/mL
Research Discipline	Inflammation
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	IL1RA
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Calibration Range	2 pg/mL to 2000 pg/mL
Interassay CV	<12%
Gene Symbol	IL1RN
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	2-2000 pg/mL
Accession Number	P25085
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	16181
Sample Type	Plasma,Serum,Supernatant
Synonym	RP23-218B17.2, F630041P17Rik, IL-1 ra

Invitrogen™ 20-Hydroxyecdysone ELISA Kit

ELISA

Pricing and Availability
Specifications

Conjugate	HRP
Interassay CV	9%
Intraassay CV	10%
Total Assay Time	2 hr 30 min
Sample Volume	Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 12.5 μL
Assay Sensitivity	197.8 pg/mL
Storage Requirements	2-8°C
Assay Range	102.4-25000 pg/mL
Test Time	45 min
Accession Number	20 E; C27H44O7; ecdysterone
Regulatory Status	RUO
Target	20-Hydroxyecdysone
Kit Contents	Anti-IgG coated 96 well plate, Standard, Assay Buffer Concentrate, Capture Antibody, HRP-conjugated Antigen, Wash Buffer Concentrate, TMB Substrate, Stop Solution, Plate Sealer,Standard,Assay Buffer Concentrate,Capture Antibody,HRP-conjugated Antigen,Wash Buffer Concentrate,TMB Substrate,Stop Solution,Plate Sealer
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Sample Type	Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye	HRP

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