missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Réactifs et kits d’immunoessais
  4. Kits ELISA de recherche en sciences de la vie
  5. Invitrogen™ Human Thrombospondin 1 ELISA Kit

Invitrogen™ Human Thrombospondin 1 ELISA Kit

Numéro de catalogue. EHTHBS1
Encompass
Modifier l'affichage
Click to view available options
Quantité:
96 Tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Numéro de catalogue. Quantité
EHTHBS1 96 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Numéro de catalogue. EHTHBS1 Fournisseur Invitrogen™ Code fournisseur EHTHBS1

Description

ELISA

The Human Thrombospondin 1 ELISA quantitates Hu Thrombospondin 1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Thrombospondin 1. Principle of the method The Human Thrombospondin 1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Spécifications

Numéro d'enregistrement P07996
Plage de l'essai 6-2000 ng/mL
Sensibilité d’essai 6 ng/mL
Plage d’étalonnage 6 ng/mL to 2000 ng/mL
Contenu et stockage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Essai Solid-phase sandwich ELISA
Conjugué HRP
Format de la trousse d’immunodosage Sandwich ELISA Kit
Type de produit ELISA
Famille de protéines Growth Factors & Receptors
Type d’échantillon Plasma, Serum, Supernatant
Spécificité This ELISA pair antibody detects human Thrombospondin-1. It shows less than 5% cross-reactivity with recombinant human Thrombospondin-2 and recombinant human Thrombospondin-4.
Suffisant pour 96 assays
Espèces cibles Human
À utiliser avec (application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène glycoprotein G; tbsp1; THBS; Thbs1; Thbs-1; thrombospondin; thrombospondin 1; thrombospondin-1; thrombospondin-1p180; thrombospondin-p50; ThrombospondinTHBS1; TSP; TSP1; TSP-1glycoprotein G; tbsp1; THBS; Thbs1; Thbs-1; thrombospondin; thrombospondin 1; thrombospondin-1; thrombospondin-1p180; thrombospondin-p50; ThrombospondinTHBS1; TSP; TSP1; TSP-1; THBS; THBS-1; TSP; TSP-1; TSP1
ID du gène (Entrez) 7057
Symbole de gène THBS1
CV intra-essai <12%, <10%
Contenus des kits Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant Biotin
Quantité 96 Tests
Statut réglementaire RUO
Disciplines de recherche Cardiovascular Research
Volume d’échantillon Plasma 10 μL, Serum 10 μL, Supernatant 100 μL
Shipping condition (Condition d'expédition) Wet or Dry Ice
Conditions de stockage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonyme THBS, THBS-1, TSP, TSP-1, TSP1
Cible Thrombospondin 1
Durée du test 1 hr 20 min
Durée totale de l'essai 4 hr 45 min
Afficher plus de résultats Afficher moins de résultats