Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit
Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).
Supplier: Thermo Scientific™ 0015159
Thermo Scientific™ QuantaRed Enhanced Chemifluorescent HRP Substrate is the most sensitive fluorescent ELISA substrate, providing sensitivity comparable to enhanced chemiluminescence.QuantaRed Enhanced Chemifluorescent HRP Substrate enables high-yield and sensitive fluorescent and colorimetric detection of peroxidase activity in the ELISA format. QuantaRed Substrate uses a proprietary technology to increase fluorescent yield and sensitivity of the well-documented ADHP (10-acetyl-3,7-dihydroxyphenoxazine) chemifluorescence reaction. ADHP is a non-fluorescent compound that reacts with horseradish peroxidase (HRP) to produce resorufin, a soluble, highly fluorescent reaction product (excitation/emission approx. 570/585nm). The long wavelength emission of the resorufin minimizes interference from the low wavelength (blue and green) autofluorescence, making it an ideal substrate for quantitating target molecules in biological samples. In addition, the colored reactopm product of QuantaRed Substrate can be measured spectrophotmetrically at 570nm.
Sensitive – low picogram to femtogram detection
Red-shifted – less interference from autofluorescence generated by biological samples
Convenient – compatible with existing filter sets, no need to purchase special filter sets
Complete – easy to use; no buffers to prepare or reagent powders to dissolve
Flexible – effective stop solution result in stable signal for at least 4 hours
Versatile – produces strong signal for easy fluorometric or colorimetric measurement
Equilibrate all reagents to room temperature before use. Temperature can affect the reaction kinetics.
|HRP Substrate Kit|
|HRP (Horseradish Peroxidase)|
|Sufficient For: 960 wells (10 x 96-well plates) at 100 µL working solution per well
• QuantaRed ADHP Concentrate, 1 mL
• QuantaRed Enhancer Solution, 50 mL
• QuantaRed Stable Peroxide Solution, 50 mL
• QuantaRed Stop Solution, 10 mL
Store at 4°C.