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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (3,999)
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1

Invitrogen™ SARS-CoV-2 Neutralizing Ab ELISA Kit  DFS Item

ELISA

Shipping Condition Wet Ice
Content And Storage 2°C to 8°C
Host Species Human
Conjugate HRP
Interassay CV <10%
Intraassay CV <10
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 5 μL, Serum 5 μL
Protein SARS-CoV-2 Spike Protein RBD
Test Time 40 mins
Regulatory Status RUO
Target SARS-CoV-2 Spike Protein RBD
Kit Contents Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum
2

Invitrogen™ Human IL-10RB ELISA Kit  DFS Item

ELISA

Protein Family Cytokines & Receptors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 5 pg/mL
Research Discipline Signal Transduction
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target IL10RB
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 5 pg/mL to 3000 pg/mL
Interassay CV <12%
Gene Symbol CDW210B,CRF2-4,CRFB4,D21S58,D21S66,IL-10R2
Storage Requirements 2°C to 8°C
Assay Range 4.12 to 3000 pg/mL
Accession Number Q08334
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 3588
Sample Type Plasma,Serum,Supernatant
Synonym CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2
4

Invitrogen™ Mouse M-CSF (CSF1) ELISA Kit  DFS Item

ELISA

Protein Family Growth Factors & Receptors
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 1 pg/mL
Research Discipline Development
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target M-CSF
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 1 pg/mL to 350 pg/mL
Interassay CV <12%
Gene Symbol C87615,Csfm,MCSF,op
Storage Requirements 2°C to 8°C
Assay Range 1.43 to 350 pg/mL
Accession Number P07141
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 12977
Sample Type Plasma,Serum,Supernatant
Synonym C87615, Csfm, MCSF, op
5

Invitrogen™ Mouse E-Cadherin (CDH1) ELISA Kit  DFS Item

ELISA

Protein Family Proteases, Peptidases & Inhibitors
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Assay Sensitivity 6 pg/mL
Research Discipline Development
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target E-cadherin
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 6 pg/mL to 3000 pg/mL
Interassay CV <12%
Gene Symbol AA960649,ARC-1,E-cad,Ecad,L-CAM,UVO,Um
Storage Requirements 2°C to 8°C
Assay Range 4.12 to 3000 pg/mL
Accession Number P09803
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 12550
Sample Type Plasma,Serum,Supernatant
Synonym AA960649, E-cad, Ecad, L-CAM, UVO, Um
6

Invitrogen™ Human Cu/ZnSOD ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Interassay CV 5.8%
Intraassay CV 5.1
Gene Symbol ALS,ALS1,HEL-S-44,IPOA,SOD,hSod1,homodimer
Total Assay Time 1 hrs 10 mins
Sample Volume Amniotic Fluid 10 μL, Fetal Umbilical Vein Blood 10 μL, Plasma 10 μL, Serum 10 μL, Supernatant 10 μL, Urine 10 μL
Assay Sensitivity 0.04 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.08 to 5.0 ng/mL
For Use With (Application) ELISA
Test Time 1 hrs 20 mins
Accession Number P00441
Regulatory Status RUO
Target SOD1
Kit Contents Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 6647
Sample Type Amniotic Fluid,Fetal Umbilical Vein Blood,Plasma,Serum,Supernatant,Urine
Label or Dye HRP
Synonym Cu/Zn Superoxide Dismutase
7

Invitrogen™ Human IL-15 ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Interassay CV 9.7%
Intraassay CV 9.3
Gene Symbol IL-15
Total Assay Time 3 hrs 30 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 3.4 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 15.6 to 1,000.0 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 20 mins
Accession Number P40933
Regulatory Status RUO
Target IL-15
Kit Contents Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3600
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin-15
8

Invitrogen™ Canine IL-2 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol IL2
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.4 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.4 to 100 ng/mL
Test Time 1 hrs 20 mins
Accession Number Aldesleukin; cytokine; H-IL-2; il 2; Il2; IL-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; Interleukin2; interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; TCGF
Regulatory Status RUO
Target IL-2
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 403989
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
9

Invitrogen™ Human IL-17AF ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Interassay CV 5.2%
Intraassay CV 5.9
Gene Symbol CANDF6,IL-17 F,ML-1,ML1,Ctla-8,Ctla8,IL-17,IL-17 A,Il17
Total Assay Time 3 hrs 30 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 8.8 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 31.3 to 2,000 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 20 mins
Accession Number Q16552,Q96PD4
Regulatory Status RUO
Target IL-17 AF
Kit Contents Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 112744, 3605
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin 17 AF, IL-17 A/F heterodimer
10

Invitrogen™ Mouse IgG (Total) Uncoated ELISA Kit

ELISA

Target Species Mouse
Conjugate HRP
Total Assay Time 24 hrs 30 mins
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity 1.56 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 1.56 to 100 ng/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Regulatory Status RUO
Target Mouse IgG
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Immunoglobulin G, Immunoglobulin G total, IgG-total
11

Invitrogen™ Human IL-8 Uncoated ELISA Kit with Plates

ELISA

Target Species Human
Conjugate HRP
Gene Symbol GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
Total Assay Time 24 hrs 30 mins
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 2 to 250 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Accession Number P10145
Regulatory Status RUO
Target IL-8
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3576
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin-8, CXCL8, NAP-I
12

Invitrogen™ Human IL-8 Uncoated ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Gene Symbol GMCSF
Total Assay Time 24 hrs 30 mins
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 2 to 250 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Accession Number P10145
Regulatory Status RUO
Target IL-8
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3576
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interleukin-8, CXCL8, NAP-I
13

Invitrogen™ Human IgG (Total) Uncoated ELISA Kit

ELISA

Target Species Human
Conjugate HRP
Total Assay Time 24 hrs 30 mins
Sample Volume Plasma 10 μL, Serum 10 μL
Assay Sensitivity 1.6 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 1.6 to 100 ng/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Regulatory Status RUO
Target Human IgG
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum
Label or Dye HRP
Synonym Immunoglobulin G
14

Invitrogen™ Human IFN gamma Uncoated ELISA Kit with Plates

ELISA

Target Species Human
Conjugate HRP
Gene Symbol CSIF,IL-10
Total Assay Time 24 hrs 30 mins
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 4 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 4 to 500 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Accession Number P01579
Regulatory Status RUO
Target IFN gamma
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3458
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Interferon-gamma, IFN-g, IFNg
15
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Invitrogen™ Rabbit IL-17A ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol IL17A
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.2 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.2 to 50 ng/mL
Test Time 1 hrs 20 mins
Accession Number CTLA8; Ctla-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; Il17a; IL-17 A; ILN; Interleukin; interleukin 17; Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17 A; interleukin 17 A-like; interleukin-17; Interleukin17A; interleukin-17 A; LOC100339322; R-IL-17-A
Regulatory Status RUO
Target IL-17 A
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 100339322
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP