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  5. Invitrogen™ Human PSA (Total)/KLK3 ELISA Kit

Invitrogen™ Human PSA (Total)/KLK3 ELISA Kit

Numéro de catalogue. EHKLK3T
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96 Tests
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Numéro de catalogue. Quantité
EHKLK3T 96 Tests
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Numéro de catalogue. EHKLK3T Fournisseur Invitrogen™ Code fournisseur EHKLK3T

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Description

ELISA

The Human Total Prostate Specific Antigen/Kallikrein 3 (Hu total PSA/KLK3) ELISA quantitates Hu total PSA/KLK3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu total PSA/KLK3. Principle of the method The Human total PSA/KLK3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and is abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. PSA is used to confirm prostatic acinar cell origin in primary and metastatic carcinoma and to rule out non-prostatic carcinoma mimics.

Spécifications

Numéro d'enregistrement P07288
Plage de l'essai 8-2000 pg/mL
Sensibilité d’essai 8 pg/mL
Plage d’étalonnage 8 pg/mL to 2000 pg/mL
Contenu et stockage Pre-coated 96-well plate, standard, detector antibody, streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers. May be stored at 2–8°C for up to 6 months from the date of shipment.
Essai Solid-phase sandwich ELISA
Conjugué HRP
Format de la trousse d’immunodosage Sandwich ELISA Kit
Type de produit ELISA
Famille de protéines Other Proteins
Type d’échantillon Plasma, Serum, Supernatant
Spécificité This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Suffisant pour 96 Assays
Espèces cibles Human
À utiliser avec (application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène Antigen; antigen, prostate specific; APS; Fletcher factor; gamma-seminoprotein; hK3; Kal3; Kal-3; Kallikrein 3; kallikrein 3, plasma; kallikrein B, plasma 1; kallikrein related peptidase 3; kallikrein-3; Kallikrein-3 (KLK3); kallikrein-related peptidase 3; Kalp1; KALP15; Kininogenin; KLK2A1; Klk3; KLK-3; Klkb1; P-30 antigen; Pk; Plasma kallikrein; Plasma kallikrein heavy chain; Plasma kallikrein light chain; Plasma prekallikrein; prostate specific antigen; prostate-specific (APS); prostate-specific antigen; PSA; Semenogelase; semininAntigen; antigen, prostate specific; APS; Fletcher factor; gamma-seminoprotein; hK3; Kal3; Kal-3; Kallikrein 3; kallikrein 3, plasma; kallikrein B, plasma 1; kallikrein related peptidase 3; kallikrein-3; Kallikrein-3 (KLK3); kallikrein-related peptidase 3; Kalp1; KALP15; Kininogenin; KLK2A1; Klk3; KLK-3; Klkb1; P-30 antigen; Pk; Plasma kallikrein; Plasma kallikrein heavy chain; Plasma kallikrein light chain; Plasma prekallikrein; prostate specific antigen; prostate-specific (APS); prostate-specific antigen; PSA; Semenogelase; seminin;
ID du gène (Entrez) 354
Symbole de gène KLK3
CV intra-essai <12%, <10%
Contenus des kits Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant Biotin
Alias des protéines PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase
Quantité 96 Tests
Statut réglementaire RUO
Disciplines de recherche Angiogenesis
Volume d’échantillon Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Shipping condition (Condition d'expédition) Wet or Dry Ice
Conditions de stockage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonyme APS, KLK2A1, PSA, hK3
Cible PSA
Durée du test 1 hr 20 min
Durée totale de l'essai 4 hr 45 min
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