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  5. Invitrogen™ Porcine TGF alpha ELISA Kit

Invitrogen™ Porcine TGF alpha ELISA Kit

Numéro de catalogue. ES21RBX5
Encompass
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5 x 96 Tests
96 Tests
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Numéro de catalogue. Quantity
ES21RBX5 5 x 96 Tests
ES21RB 96 Tests
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Numéro de catalogue. ES21RBX5 Fournisseur Invitrogen™ Code fournisseur ES21RBX5
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Description

ELISA

Porcine TGF alpha quantitates porcine TGF alpha in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant porcine TGF alpha.

TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Spécifications

Accession Number EGF-like TGF; ETGF; LOC100009482; protransforming growth factor alpha; RATTGFAA; TFGA; TGF a; TGF type 1; TGF a; Tgfa; TGFAA; TGFalpha; TGF-alpha; TGFa; Transforming growth factor; Transforming growth factor alpha; transforming growth factor, alpha; transforming growth factor-alpha; wa1; wa-1; waved 1
Assay Range 0.6 to 120 pg/mL
Assay Sensitivity 0.6 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 397484
Gene Symbol TGFA
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target TGF alpha
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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