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![Invitrogen Multispecies AKT (Phospho) [pS473] InstantOne ELISA Kit:Immunoassay](https://assets.fishersci.com/TFS-Assets/BID/product-images/TGR.ELISA.Kit-650x600.jpg-650.jpg)
Invitrogen™ Multispecies AKT (Phospho) [pS473] InstantOne™ ELISA Kit
ELISA
$966.00
Spécifications
| Conjugate | HRP |
|---|---|
| Product Type | ELISA |
| Sample Type | Cell Lysates |
| For Use With (Application) | ELISA |
| For Use With (Equipment) | Colorimetric Microplate Reader |
Comprend: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
phospho-AKT 1/2/3 (Ser473) Capture Antibody Reagent
phospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate seal
| Numéro de catalogue | Numéro du manufacturier. | Quantity | Prix | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Numéro de catalogue | Numéro du manufacturier. | Quantity | Prix | Quantity & Availability | |||||
|
50246243
|
Invitrogen™
858604211 |
96 Tests |
chaque for $966.00
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Description
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster AKT 1/2/3 in cell lysates. Detection of rat AKT 1/2/3 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.Spécifications
| HRP | |
| Cell Lysates | |
| Colorimetric Microplate Reader | |
| Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal | |
| Cell Lysate 20 μL | |
| PKB, Protein Kinase B, RAC-Pk.-alpha | |
| 1 hrs 20 mins |
| ELISA | |
| ELISA | |
| 207 | |
| RUO | |
| 2°C to 8°C | |
| AKT1 | |
| 1 hrs 10 mins |