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  5. Invitrogen™ Multispecies AKT (Phospho) [pS473] InstantOne™ ELISA Kit

Invitrogen™ Multispecies AKT (Phospho) [pS473] InstantOne™ ELISA Kit

Catalog No. 50246243
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96 Tests
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Catalog No. Quantity
50246243 96 Tests
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Catalog No. 50246243 Supplier Invitrogen™ Supplier No. 858604211
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Description

Includes

User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
phospho-AKT 1/2/3 (Ser473) Capture Antibody Reagent
phospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate seal

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster AKT 1/2/3 in cell lysates. Detection of rat AKT 1/2/3 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

Specifications

Accession Number P31749
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 207
Gene Symbol AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,MPPH,MPPH2,PKB-GAMMA,PKBG,PRKBG,RAC-Pk.-gamma,RAC-gamma,STK-2
Kit Contents Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym PKB, Protein Kinase B, RAC-Pk.-alpha
Target AKT1
Test Time 1 hrs 20 mins
Total Assay Time 1 hrs 10 mins
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